Genomic Variants, Transcriptomic Profile, Ultrasonographic Findings, and Antioxidant and Immunological Biomarkers Linked to Pregnancy Toxemia Susceptibility in Goats.

Pregnancy toxemia (PT) represents a significant metabolic disorder affecting small ruminants that causes substantial economic losses due to reduced productivity, reproductive failure, and high mortality. This study investigated the clinical, ultrasonographic, hematobiochemical, oxidative stress, and immunological profiles, as well as the gene expression and nucleotide sequence variations, associated with PT susceptibility in Shami goats. Fifty late-pregnant does (33 healthy and 17 PT-affected) were examined. Clinical evaluation, complete blood count, biochemical analysis, cytokine profiling, antioxidant assays, hepatic ultrasonography, quantitative real-time PCR of immune (IL6, IL8), antioxidant (SOD3, HMOX1), and lipogenic (ACACA, FASN) genes, and PCR-DNA sequencing were performed. PT does exhibit significant hypoglycemia, hyperketonemia, elevated liver and kidney function biomarkers, dyslipidemia, oxidative stress (↑ MDA, ↓ GSH, GPx, SOD, CAT), increased pro-inflammatory cytokines (IL1α, IL1β, IL6, TNFα), and reduced IL10. Gene expression analysis revealed upregulation of IL6 and IL8 and downregulation of SOD3, HMOX1, ACACA, and FASN in PT does. Sequencing identified multiple synonymous and non-synonymous SNPs significantly associated with PT. Ultrasonography indicated hepatic fatty infiltration. Discriminant analysis using SNPs achieved 100% classification accuracy between healthy and PT does. These findings suggested that combined clinical, biochemical, oxidative, immunological, and genetic markers could enhance early PT diagnosis and may provide a basis for future studies aimed at selective breeding for improved resistance.