Evidence map›Paper›PMID 41011768›Full record

ArticlePathogens (Basel, Switzerland)2025

Reverse Transcription Recombinase-Aided Amplification Assay for Newcastle Disease Virus in Poultry.

Nahed Yehia, Ahmed Abd El Wahed, Ahmed Abd Elhalem Mohamed, Abdelsattar Arafa, Dalia Said, Mohamed A Shalaby, Arianna Ceruti, Uwe Truyen, Rea Maja Kobialka

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Article in Pathogens (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Nahed YehiaReference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center, Dokki, Giza 12618, Egypt.ORCID 0000-0002-2823-6467
Ahmed Abd El WahedInstitute of Animal Hygiene and Veterinary Public Health, Leipzig University, 04109 Leipzig, Germany.ORCID 0000-0003-3347-6075
Ahmed Abd Elhalem MohamedReference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center, Dokki, Giza 12618, Egypt.
Abdelsattar ArafaReference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center, Dokki, Giza 12618, Egypt.
Dalia SaidReference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center, Dokki, Giza 12618, Egypt.
Mohamed A ShalabyDepartment of Virology, Faculty of Veterinary Medicine, Cairo University, Cairo 12211, Egypt.
Arianna CerutiInstitute of Animal Hygiene and Veterinary Public Health, Leipzig University, 04109 Leipzig, Germany.ORCID 0009-0002-3219-8691
Uwe TruyenInstitute of Animal Hygiene and Veterinary Public Health, Leipzig University, 04109 Leipzig, Germany.
Rea Maja KobialkaInstitute of Animal Hygiene and Veterinary Public Health, Leipzig University, 04109 Leipzig, Germany.ORCID 0009-0007-5114-1910

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Newcastle disease (ND) is a highly contagious and economically significant viral infection that affects poultry globally, with recurrent outbreaks occurring even among vaccinated flocks in Egypt. Caused by the Newcastle disease virus (NDV), the disease results in substantial losses due to high mortality rates, decreased productivity, and the imposition of trade restrictions. This study aimed to develop a rapid, sensitive, and field-deployable diagnostic assay based on real-time reverse transcription recombinase-aided amplification (RT-RAA) for the detection of all NDV genotypes in clinical avian specimens. Primers and an exo-probe were designed based on the most conserved region of the NDV matrix gene. After testing ten primer combinations, the pair NDV RAA-F1 and RAA-R5 demonstrated the highest sensitivity, detecting as low as 6.89 EID

Indexed as

Newcastle DiseaseNewcastle disease virusNucleic Acid Amplification TechniquesPoultry DiseasesRecombinasesAnimalsChickensDNA PrimersEgyptGenotypePoultryReverse TranscriptionSensitivity and SpecificityDNA PrimersRecombinasesNDVrapid diagnosticsRT-PCRRT-RAA

Identifiers

PMID41011768
PMCPMC12473048

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.