Evidence map›Paper›PMID 41011356›Full record

ArticleMicroorganisms2025

The Trans-Kingdom Spectrum of Mpox-like Lesion Pustules of Suspect Patients in the Mpox Clade Ib Outbreak in Eastern Democratic Republic of the Congo.

Leandre Murhula Masirika, Benjamin Hewins, Ali Toloue Ostadgavahi, Mansi Dutt, Léandre Mutimbwa Mambo, Jean Claude Udahemuka, Pacifique Ndishimye, Justin Bengehya Mbiribindi, Freddy Belesi Siangoli, Patricia Kelvin and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microorganisms, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Observational
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Leandre Murhula MasirikaCentre de Recherche en Sciences Naturelles de Lwiro, DS Bukavu, Sud-Kivu, Democratic Republic of the Congo.ORCID 0009-0000-0688-5688
Benjamin HewinsDepartment of Microbiology and Immunology, Canadian Center for Vaccinology (CCfV), Faculty of Medicine, Dalhousie University, Halifax, NS B3H 4R2, Canada.ORCID 0009-0003-0941-5720
Ali Toloue OstadgavahiDepartment of Microbiology and Immunology, Canadian Center for Vaccinology (CCfV), Faculty of Medicine, Dalhousie University, Halifax, NS B3H 4R2, Canada.
Mansi DuttDepartment of Microbiology and Immunology, Canadian Center for Vaccinology (CCfV), Faculty of Medicine, Dalhousie University, Halifax, NS B3H 4R2, Canada.
Léandre Mutimbwa MamboZone de Santé de Kamituga, Kamituga, Sud-Kivu, Democratic Republic of the Congo.
Jean Claude UdahemukaDepartment of Veterinary Medicine, University of Rwanda, Nyagatare P.O. Box 57, Rwanda.ORCID 0000-0001-8370-7113
Pacifique NdishimyeStansile Research Organization, Kigali P.O. Box 5377, Rwanda.ORCID 0000-0003-3285-2622
Justin Bengehya MbiribindiDivision Provinciale de la Santé, DS Bukavu, Sud-Kivu, Democratic Republic of the Congo.
Freddy Belesi SiangoliDivision Provinciale de la Santé, DS Bukavu, Sud-Kivu, Democratic Republic of the Congo.
Patricia KelvinDepartment of Microbiology and Immunology, Canadian Center for Vaccinology (CCfV), Faculty of Medicine, Dalhousie University, Halifax, NS B3H 4R2, Canada.
Morgan G I LangilleDepartment of Pharmacology, Dalhousie University, Halifax, NS B3H 4R2, Canada.
David J KelvinDepartment of Microbiology and Immunology, Canadian Center for Vaccinology (CCfV), Faculty of Medicine, Dalhousie University, Halifax, NS B3H 4R2, Canada.
Luis FloresCentre de Recherche en Sciences Naturelles de Lwiro, DS Bukavu, Sud-Kivu, Democratic Republic of the Congo.ORCID 0000-0002-0089-4716
Gustavo Sganzerla MartinezDepartment of Microbiology and Immunology, Canadian Center for Vaccinology (CCfV), Faculty of Medicine, Dalhousie University, Halifax, NS B3H 4R2, Canada.ORCID 0000-0002-7656-0579
Anuj KumarDepartment of Microbiology and Immunology, Canadian Center for Vaccinology (CCfV), Faculty of Medicine, Dalhousie University, Halifax, NS B3H 4R2, Canada.ORCID 0000-0002-5023-7618

Funding

CIHR CIHR MZ1 187236Dalhousie Medical Research Foundation N/ALi-Ka Shing Foundation N/AResearch Nova Scotia 2023-2565
6 · The paper itself

Abstract

During infectious disease outbreaks, acquiring genetic data across various kingdoms offers essential information to tailor precise treatment methodologies and bolster clinical, epidemiological, and public health awareness. Metagenomics sequencing has paved the way for personalized treatment approaches and streamlined the monitoring process for both co-infections and opportunistic infections. In this study, we conducted long-read metagenomic DNA sequencing on mpox-like lesion pustules from six suspected patients who were positive and confirmed to be infected with MPXV during the MPXV subclade Ib outbreak in the Eastern Democratic Republic of the Congo. The sequenced data were taxonomically classified as bacterial, fungal, and viral in composition. Our results show a wide spectrum of microorganisms present in the lesions. Bacteria such as

Indexed as

clade Ibco-infectionmetagenomicsmpox

Identifiers

PMID41011356
PMCPMC12472868

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.