Impact of PGT Introduction on IVF Laboratory Workload: Lessons Learned from a Single-Center Experience of 5258 Biopsies over a 10-Year Period.

The aim of our study was to provide a retrospective single-center experience of the additional workload associated with routine PGT, including embryologist training and suggested staffing levels. A total of 4945 IVF cycles were retrospectively considered, of which 1680 were PGT cycles with a total of 5258 biopsied blastocysts. An exponential increase in the proportion of PGTs over OPUs was observed, from 0.2% in 2015 to 72.9% in 2024. The number of viable embryos for biopsy was significantly increased by the systematic adoption of an extended embryo culture and the concomitant transition from a day 2 Double Embryo Transfer (DET) to a day 5 Single Blastocyst Transfer (SET) policy in 2020. In order to cope with the increasing workload, a concomitant increase in the number of embryologists involved in blastocyst biopsy was adopted, with a second embryologist in 2020, a third in 2021, and a fourth in 2022, with a trend comparable to that observed for the proportion of PGT cycles over IVF cycles performed during the study period. The appropriate number of staff required for the IVF laboratory was calculated using the Staffing Model for ART (smART) calculator, based on 12 routine IVF procedures. An optimal balance between operational procedures and staffing levels was achieved when the difference (Δ) was ≤10%, ensuring the efficient maintenance of PGT in the IVF laboratory.