Evidence map›Paper›PMID 41010281›Full record

ArticleLife (Basel, Switzerland)2025

FGF21-MAPK1 Imbalance Disrupts Hepatic Lipid Metabolism in Dairy Cow Ketosis.

Jun-Jie Xu, Fan Yang, Zhi-Xi Chen, Zhi-Peng Wang, Zi-Xuan Wang, Zi-Han Deng, Chen-Jie Xu, Fang-Hui Chen, Wei Zhang, Yang Liu and 1 more

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Article in Life (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Jun-Jie XuCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Fan YangDepartment of Human Anatomy, Bengbu Medical University, Bengbu 233030, China.
Zhi-Xi ChenCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Zhi-Peng WangCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Zi-Xuan WangCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Zi-Han DengCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Chen-Jie XuCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Fang-Hui ChenCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Wei ZhangCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.ORCID 0000-0002-8359-6668
Yang LiuCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Ya-Fei CaiCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

Funding

National Science and Technology Innovation Project 2030 2023ZD0404802
6 · The paper itself

Abstract

backgroundAberrant hepatic lipid metabolism is a key predisposing factor for dairy cow ketosis, with genetic factors playing a pivotal role in disease pathogenesis. However, systematic screening and functional validation of candidate genes for bovine ketosis remain limited. In this study, we aimed to identify genetic markers associated with clinical ketosis and explore their potential functional mechanisms underlying disease susceptibility.

methodsWe conducted simplified genome sequencing (SuperGBS), genome-wide association studies (GWAS), and Sanger sequencing on Chinese Holstein cows, both healthy and with ketosis.

resultsWe reported that mitogen-activated protein kinase 1 (MAPK1) was significantly associated with clinical ketosis. Further investigation revealed concurrent upregulation of MAPK1 protein and disrupted hepatic lipid homeostasis in hepatocytes from in vivo and in vitro models. Critically, siRNA-mediated knockdown of MAPK1 reversed lipid metabolism processes and reduced lipid accumulation in β-Hydroxybutyric acid (BHB)-exposed bovine hepatocytes, thereby establishing MAPK1 activation as a driver of lipotoxicity in dairy cow ketosis. Additionally, we identified that supplementation of fibroblast growth factor 21 (FGF21) fusion protein not only reduced MAPK1 expression but also normalized hepatic lipid metabolism in BHB-exposed bovine hepatocytes.

conclusionsFGF21-MAPK1 imbalance is a reason for hepatic lipid metabolic dysfunction, providing a potential intervention approach to mitigate dairy cows' ketosis.

Indexed as

dairy cowsFGF21ketosislipid metabolismMAPK1

Identifiers

PMID41010281
PMCPMC12470934

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.