Evidence map›Paper›PMID 41009978›Full record

ArticleGenes2025

Sex-Specific Transcriptome Signatures in Pacific Oyster Hemolymph.

Jingwei Song, Odile V J Maurelli, Mark S Yeats, Neil F Thompson, Michael A Banks, Bernarda Calla

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jingwei SongDepartment of Fisheries, Wildlife and Conservation Sciences, Coastal Oregon Marine Experimental Station (COMES), Hatfield Marine Science Center, Oregon State University, Newport, OR 97365, USA.
Odile V J MaurelliDepartment of Fisheries, Wildlife and Conservation Sciences, Coastal Oregon Marine Experimental Station (COMES), Hatfield Marine Science Center, Oregon State University, Newport, OR 97365, USA.
Mark S YeatsDepartment of Fisheries, Wildlife and Conservation Sciences, Coastal Oregon Marine Experimental Station (COMES), Hatfield Marine Science Center, Oregon State University, Newport, OR 97365, USA.
Neil F ThompsonPacific Shellfish Research Unit, United States Department of Agriculture, Agricultural Research Service, Newport, OR 97365, USA.ORCID 0000-0001-7708-7667
Michael A BanksDepartment of Fisheries, Wildlife and Conservation Sciences, Coastal Oregon Marine Experimental Station (COMES), Hatfield Marine Science Center, Oregon State University, Newport, OR 97365, USA.
Bernarda CallaDepartment of Fisheries, Wildlife and Conservation Sciences, Coastal Oregon Marine Experimental Station (COMES), Hatfield Marine Science Center, Oregon State University, Newport, OR 97365, USA.ORCID 0000-0001-8684-616X

Funding

Agricultural Research Service 2076-10600-001-00DAgricultural Research Service 2076-63000-005-000-D
6 · The paper itself

Abstract

BACKGROUND/

objectivesSex determination and differentiation exhibit remarkable molecular diversity across taxa, driven by genetic, epigenetic, and environmental factors. Invertebrates with sequential hermaphroditism, such as the Pacific oyster (

methodsTo identify sex-related molecular markers during gametogenesis, we repeatedly sampled hemolymph from artificially conditioned oysters over two months, and sex phenotypes were assigned at the end of the experiment by biopsy.

resultsRNA-sequencing analysis of five males and five females revealed subtle yet consistent sex-specific transcriptional signatures in hemolymph. We show that gametogenesis proceeds asynchronously among oysters, even within the same sex individuals. Complex physiological trade-offs were discovered between sexes during gonad maturation; in early stages of sexual maturation, females prioritized cell division, whereas males suppressed it. Females exhibited higher expression of solute carrier family (SLC) genes, suggesting enhanced nutrient exchange during oogenesis. Temporal dynamics highlighted differential expression of genes regulating cross-membrane ion gradients (e.g., transient receptor potential channels) and signal transduction (e.g., signal transducer and activator of transcription), previously linked to environmental sex determination (ESD) in some reptilian species.

conclusionsTogether, these findings underscore that gametogenesis in Pacific oysters is complex and dynamic, and that molecular pathways of ESD may be partially conserved between invertebrate and vertebrate species.

Indexed as

HemolymphOstreidaeTranscriptomeAnimalsFemaleGametogenesisGene Expression ProfilingGonadsMaleOogenesisSex Determination ProcessesgametogenesishemolymphMagallana gigasoystersex determination

Identifiers

PMID41009978
PMCPMC12469842

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.