ArticleFoods (Basel, Switzerland)2025
Silver Nanocluster-Based Label-Free Aptasensor for the Turn-On Fluorescent Detection of Ochratoxin A.
Article in Foods (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Despite the substantial human health risks posed by ochratoxin A (OTA), a potent mycotoxin, simple, low-cost methods for its sensitive and selective detection in foods are lacking. To address this gap, we herein developed a label-free OTA aptasensor based on deoxyribonucleic acid (DNA)-scaffolded silver nanoclusters (AgNCs) with an intense red fluorescence. As the DNA template fragment used for AgNC fabrication was derived from the complementary sequence of the OTA aptamer (Apt-OTA), Apt-OTA complexed the AgNCs in the absence of OTA, quenching their fluorescence. OTA inhibited this quenching by strongly binding Apt-OTA and thus precluding its binding to the AgNCs. The OTA aptasensor exhibited a high selectivity and low detection limit (0.38 ng/mL), eliminating the need for expensive reagents, complicated pre-treatments, and advanced equipment, and was successfully used to quantify mycotoxins in food under real-life conditions, thus holding promise for mycotoxin control.
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