ArticleScientific reports2025
Mechanism of sepsis regulation by ELANE via macrophage polarization.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Diagnostic biomarkers for non-traumatic osteonecrosis of the femoral head : a systematic review and pathophysiological cascade network.Bone & joint research · 2026Pooled it
- Potential targets of baicalein in macrophages revealed by bulk and single cell RNA sequencing analysis.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- The multidimensional signaling of ELANE: from congenital hematopoietic failure to immune microenvironment crosstalk and targeted interventions.Cell communication and signaling : CCS · 2026Review
- Sepsis modelling: current approaches and organ-on-chip perspectives.EBioMedicine · 2026Review
- J2R/F4L deleted oncolytic vaccinia virus synergizes with tumor specific killer (ELANE) promotes antitumor immunity with superior safety.Frontiers in immunology · 2026Article
- Multi-omic profiling converges on proteasome subunits PSMA7/PSMB2 as targets of the sepsis-protective agent Handelin.Frontiers in immunology · 2026Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
To identify the core immune regulatory gene ELANE in sepsis and explore its mechanism in regulating sepsis through macrophage polarization. Peripheral blood RNA sequencing and bioinformatics analysis were performed on 20 sepsis patients and 10 healthy controls. Differential gene expression (DEGs) screening, functional enrichment (GO/KEGG/GSEA), and protein-protein interaction (PPI) network analysis were conducted. Meta-analysis validated ELANE expression trends across GEO datasets, while survival analysis assessed its prognostic relevance. Single-cell sequencing localized ELANE expression, and in vitro experiments using LPS-stimulated macrophages and siRNA-mediated ELANE inhibition evaluated its role in M1/M2 macrophage polarization and inflammatory factor release (flow cytometry, ELISA). 948 DEGs were identified, with ELANE significantly upregulated in sepsis. PPI and MCC algorithms identified ELANE as a top core gene. Meta-analysis confirmed ELANE overexpression in sepsis, and survival analysis linked high ELANE levels to poor 28-day prognosis. Single-cell sequencing localized ELANE predominantly in macrophages. LPS stimulation increased ELANE expression and M1 polarization (CD86+), while ELANE inhibition reduced M1 polarization and suppressed IL-1β/TNF-α levels. ELANE exacerbates sepsis by promoting M1 macrophage polarization and proinflammatory cytokine release, highlighting its potential as a therapeutic target.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.