Evidence map›Paper›PMID 41006618›Full record

ArticleScientific reports2025

Construction of an infectious clone of novel duck picornavirus using the infectious subgenomic amplicon method.

Jitong Li, Tong Zhu, Junfeng Lv, Yuehua Gao, Feng Hu, Kexiang Yu, Minxun Song, Jianlin Wang, Bai Wei, Min Kang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Jitong LiInstitute of Poultry Science, Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
Tong ZhuInstitute of Poultry Science, Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
Junfeng LvInstitute of Poultry Science, Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
Yuehua GaoInstitute of Poultry Science, Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
Feng HuInstitute of Poultry Science, Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
Kexiang YuInstitute of Poultry Science, Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
Minxun SongInstitute of Poultry Science, Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
Jianlin WangCollege of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, China.
Bai WeiDepartment of Avian Diseases, College of Veterinary Medicine, Center for Avian Disease, Jeonbuk National University, Iksan, 54596, Republic of Korea.
Min KangDepartment of Avian Diseases, College of Veterinary Medicine, Center for Avian Disease, Jeonbuk National University, Iksan, 54596, Republic of Korea.
Yufeng LiInstitute of Poultry Science, Shandong Provincial Key Laboratory of Immunity and Diagnosis of Poultry Diseases, Shandong Academy of Agricultural Sciences, Jinan, 250100, China. dicpd@163.com.

Funding

Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Animal Disease Management Technology Advancement Support Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) 122014-2Shandong Province Poultry Industry Technology System SDAIT-11-01Shandong Provincial Agricultural Variety Project 2022LZGC014
6 · The paper itself

Abstract

A picornavirus strain of Duck/FC22/China/2017 (FC22) with an unclear taxonomic status was isolated in our laboratory in 2017. To study the biological properties of the virus, an infectious clone of the FC22 strain was successfully rescued by the infectious subgenomic amplicon method. The FC22 gene was amplified in two segments and fused with CMV and HDR after the addition of marker sites. The fusion fragment was transfected into LMH cells and passaged, and the obtained virus was named rFC22 and identified by PCR, IFA and electron microscopy. The biological characteristics of strain rFC22 were studied by inoculation with LMH cells, and the results showed that the TCID

Indexed as

DucksGenome, ViralPicornaviridaePicornaviridae InfectionsPoultry DiseasesAnimalsCell LineBiological characteristicsDuck picornavirusInfectious cloneInfectious subgenomic amplicons

Identifiers

PMID41006618
PMCPMC12474889

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.