Evidence map›Paper›PMID 41003998›Full record

ReviewSub-cellular biochemistry2025

Effects of Molecular Crowding on the Structure, Folding, and Stability of DNA.

Victor M Golyshev, Nadezhda Yu Biziukova, Olga A Tarasova, Alexander A Lomzov

Abstract readReview
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In one paragraph

Review in Sub-cellular biochemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Victor M GolyshevLaboratory of Structural Biology, Institute of Chemical Biology & Fundamental Medicine SB RAS, Novosibirsk, Russia.
Nadezhda Yu BiziukovaDepartment of Bioinformatics, Laboratory of Big Data Analysis for Digital Pharmacology, Institute of Biomedical Chemistry, Moscow, Russia.
Olga A TarasovaDepartment of Bioinformatics, Laboratory of Big Data Analysis for Digital Pharmacology, Institute of Biomedical Chemistry, Moscow, Russia.
Alexander A LomzovLaboratory of Structural Biology, Institute of Chemical Biology & Fundamental Medicine SB RAS, Novosibirsk, Russia. lomzov@niboch.nsc.ru.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter comprehensively reviews the effects of molecular crowding on the structurе, folding, and thermodynamic stability of various DNA structures. While traditional studies often utilize dilute solutions, cellular environments are characterized by high macromolecular concentrations, ions, and metabolites, which significantly alter DNA behavior. The chapter discusses experimental methods, highlighting their strengths and limitations in studying DNA under crowded conditions. Key structural transformations-such as B-A and B-Z DNA conformational transitions, triplex formation, G-quadruplex (G4) topologies, and i-motif stabilization-are examined in the context of cosolute-induced effects. The chapter emphasizes the challenges of replicating in vivo conditions in vitro and underscores the need for innovative approaches to study nucleic acids in physiologically relevant environments. These insights advance our understanding of DNA behavior in cellular contexts, with implications for biotechnology, drug design, and gene regulation.

Indexed as

DNANucleic Acid ConformationBiochemical PhenomenaNucleotide MotifsThermodynamicsDNAA-DNAB-DNADNA foldingDNA stabilityDNA structureDNA triplexExperimental methodsG-quadruplexI-motifMolecular crowdingThermodynamicsZ-DNA

Identifiers

PMID41003998

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.