Evidence map›Paper›PMID 41003921›Full record

ArticleBioresources and bioprocessing2025

Development of an in vitro multi-enzyme system for efficient one-pot biosynthesis of sorbitol from fructose-6-phosphate.

Kai Shen, Chao-Nan Zhu, Jian-He Xu, Gao-Wei Zheng, Qi Chen

Abstract read
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In one paragraph

Article in Bioresources and bioprocessing, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kai ShenState Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, Shanghai, 200237, China.
Chao-Nan ZhuState Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, Shanghai, 200237, China.
Jian-He XuState Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, Shanghai, 200237, China.
Gao-Wei ZhengState Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, Shanghai, 200237, China. gaoweizheng@ecust.edu.cn.
Qi ChenState Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, Shanghai, 200237, China. chenqi_angela@163.com.ORCID http://orcid.org/0000-0002-1287-2557

Funding

Key Technologies Research and Development Program 2021YFC2102300，2019YFA0905000Science and Technology Commission of Shanghai Municipality 25ZR1401078, 25HC2810400, 24HC2810300 and 23HC1400200
6 · The paper itself

Abstract

Fructose-6-phosphate is a key ketose monophosphate intermediate that plays a central role in the metabolic pathway of glycolysis. Sorbitol is a naturally occurring sugar alcohol with diverse biological properties, which is widely applied in the food, cosmetic, and pharmaceutical industries due to its favorable physicochemical and physiological characteristics. Although a biosynthetic pathway from starch to fructose-6-phosphate has been well established in metabolic engineering studies, the downstream enzymatic route leading to the biosynthesis of sorbitol from this intermediate has not yet been elucidated in the literature. In this study, we performed the design and implementation of an artificial multi-enzymatic cascade system for the biosynthesis of sorbitol from fructose-6-phosphate. Firstly, candidate enzymes sorbitol-6-phosphate dehydrogenase (S6PDH) and sorbitol-6-phosphate dephosphatase (S6PDP) were selected with an initial activity of 147 U/mg and 1.7 U/mg, respectively. Secondly, molecular modifications were then carried out to improve the thermostability of EcS6PDH, EcS6PDH-M4 displays markedly improved thermostability, with a significant extension of half-life at 40 °C from less than 1 min to 375 min and an elevated T

Indexed as

Fructose-6-phosphateMulti-enzymatic systemProtein engineeringSorbitolSorbitol-6-phosphate dehydrogenaseSorbitol-6-phosphate dephosphatase

Identifiers

PMID41003921
PMCPMC12474828

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.