Evidence map›Paper›PMID 41003759›Full record

ArticleMetabolomics : Official journal of the Metabolomic Society2025

OMNImet®•GUT collection kit supports sample collection and ambient temperature preservation of faecal short chain fatty acids.

Jemma Cleminson, Caroline Kerbiriou, Shona McKirdy, Lauren Beck, David I Campbell, Andrew R Gennery, Janet E Berrington, Konstantinos Gerasimidis, Christopher J Stewart

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In one paragraph

Article in Metabolomics : Official journal of the Metabolomic Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

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No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Jemma CleminsonTranslational and Clinical Research Institute, Faculty of Medical Sciences, The Medical School, Newcastle University, Framlington Place, Newcastle Upon Tyne, NE2 4HH, UK. jemma.cleminson@newcastle.ac.uk.
Caroline KerbiriouHuman Nutrition, School of Medicine, Dentistry & Nursing, University of Glasgow, Glasgow Royal Infirmary, New Lister Building, Glasgow, G31 2ER, UK.
Shona McKirdyHuman Nutrition, School of Medicine, Dentistry & Nursing, University of Glasgow, Glasgow Royal Infirmary, New Lister Building, Glasgow, G31 2ER, UK.
Lauren BeckTranslational and Clinical Research Institute, Faculty of Medical Sciences, The Medical School, Newcastle University, Framlington Place, Newcastle Upon Tyne, NE2 4HH, UK.
David I CampbellDepartment of Paediatric Gastroenterology, Great North Children's Hospital, Royal Victoria Infirmary, Tyne and Wear, Newcastle Upon Tyne, NE1 4LP, UK.
Andrew R GenneryTranslational and Clinical Research Institute, Faculty of Medical Sciences, The Medical School, Newcastle University, Framlington Place, Newcastle Upon Tyne, NE2 4HH, UK.
Janet E BerringtonTranslational and Clinical Research Institute, Faculty of Medical Sciences, The Medical School, Newcastle University, Framlington Place, Newcastle Upon Tyne, NE2 4HH, UK.
Konstantinos GerasimidisHuman Nutrition, School of Medicine, Dentistry & Nursing, University of Glasgow, Glasgow Royal Infirmary, New Lister Building, Glasgow, G31 2ER, UK.
Christopher J StewartTranslational and Clinical Research Institute, Faculty of Medical Sciences, The Medical School, Newcastle University, Framlington Place, Newcastle Upon Tyne, NE2 4HH, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionShort-chain fatty acids (SCFAs) are microbial metabolites that play critical roles in health. As SCFAs are highly volatile molecules and ongoing bacterial metabolism in faecal samples collected can cause compositional shifts, quick SCFA preservation at the point of sample collection is of utmost importance for valid results.

objectivesThis study aimed to evaluate the OMNImet®•GUT kit as an immediate preservation method that stabilises SCFAs at room temperature compared to a standardised laboratory sodium hydroxide method.

methodsFaecal samples from 12 participants were included. For each sample, 1 g faeces was aliquoted into 2.5 ml OMNImet®•GUT buffer and 1 g faeces was aliquoted into 2.5 ml sodium hydroxide (current laboratory method). SCFA profiles were quantified at zero, 24-, and 48-h using gas chromatography. SCFA profiles were compared across patients, preservation method, and time point.

resultsResults showed that most SCFA profiles were stable and comparable between methods. The sample donor explained the most variance and was the most significant factor associated with the SCFA profiles, independent of the collection kit or duration at room temperature (R

conclusionThese findings support OMNImet®•GUT as a practical alternative for SCFA preservation, particularly for multicentre studies where immediate sample collection can be logistically challenging, but it is important to use a consistent collection method within studies.

Indexed as

Fatty Acids, VolatileFecesSpecimen HandlingAdultAgedFemaleHumansMaleMiddle AgedTemperatureFatty Acids, VolatileHumanMicrobiomeOMNImet®•GUTShort chain fatty acids

Identifiers

PMID41003759

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.