Evidence map›Paper›PMID 41003751›Full record

ArticleAnalytical and bioanalytical chemistry2025

Characterization of rare trifucosylated human milk oligosaccharides by cryogenic infrared ion spectroscopy (CIRIS).

Ali H Abikhodr, Stephan Warnke, Ahmed Ben Faleh, Thomas R Rizzo, Sibel Goeraler, John Gonsalves, Bernd Stahl, Marko Mank

Abstract read
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In one paragraph

Article in Analytical and bioanalytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ali H AbikhodrIsospec Analytics SA, CH-1020, Renens, Switzerland.
Stephan WarnkeIsospec Analytics SA, CH-1020, Renens, Switzerland.
Ahmed Ben FalehIsospec Analytics SA, CH-1020, Renens, Switzerland.
Thomas R RizzoIsospec Analytics SA, CH-1020, Renens, Switzerland. thomas.rizzo@epfl.ch.ORCID http://orcid.org/0000-0003-2796-905X
Sibel GoeralerDanone Research and Innovation, 3584 CT, Utrecht, The Netherlands.
John GonsalvesDanone Research and Innovation, 3584 CT, Utrecht, The Netherlands.
Bernd StahlDanone Research and Innovation, 3584 CT, Utrecht, The Netherlands.
Marko MankDanone Research and Innovation, 3584 CT, Utrecht, The Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

While approximately 200 human milk oligosaccharide structures (HMOs) have been completely characterized, the precise isomeric structures of even more HMOs remain ambiguous. To address this analytical challenge, we employ tandem mass spectrometry (i.e., MS/MS) coupled with cryogenic infrared ion spectroscopy (CIRIS) to characterize and identify novel fucosylated HMOs isolated from pooled human milk. In this approach, the precise structure of isomeric precursor molecules purified by liquid chromatography (LC) is identified by IR fingerprinting their fragments. Using this technique, we have identified four novel isomers of trifucosylated oligosaccharides (i.e., trifucosyl-lacto-N-heptaoses and trifucosyl-lacto-N-neoheptaoses), some of which could not have been produced by previously known biosynthetic pathways for HMO synthesis in the mammary gland.

Indexed as

Milk, HumanChromatography, LiquidFemaleFucoseHumansOligosaccharidesSpectrophotometry, InfraredFucoseOligosaccharidesCryogenic IR spectroscopyGlycan analysisHuman milk oligosaccharidesMass spectrometryStructural analysis of glycomicsTF-LNT

Identifiers

PMID41003751
PMCPMC12583426

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.