Evidence map›Paper›PMID 41003308›Full record

ArticleMarine drugs2025

New Strategy for the Degradation of High-Concentration Sodium Alginate with Recombinant Enzyme 102C300C-Vgb and the Beneficial Effects of Its Degradation Products on the Gut Health of

Ziqiang Gu, Feiyu Niu, Peng Yang, Wenling Gong, Hina Mukhtar, Siyu Li, Yanwen Zheng, Yiling Zhong, Hanyi Cui, Jichao Li and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Marine drugs, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Ziqiang GuCollege of Food Science and Engineering, Ocean University of China, No.1299 Sansha Road, Qingdao 266003, China.
Feiyu NiuCollege of Food Science and Engineering, Ocean University of China, No.1299 Sansha Road, Qingdao 266003, China.
Peng YangCollege of Food Science and Engineering, Ocean University of China, No.1299 Sansha Road, Qingdao 266003, China.
Wenling GongCollege of Food Science and Engineering, Ocean University of China, No.1299 Sansha Road, Qingdao 266003, China.
Hina MukhtarCollege of Food Science and Engineering, Ocean University of China, No.1299 Sansha Road, Qingdao 266003, China.
Siyu LiCollege of Food Science and Engineering, Ocean University of China, No.1299 Sansha Road, Qingdao 266003, China.ORCID 0009-0007-6821-8750
Yanwen ZhengCollege of Food Science and Engineering, Ocean University of China, No.1299 Sansha Road, Qingdao 266003, China.
Yiling ZhongCollege of Food Science and Engineering, Ocean University of China, No.1299 Sansha Road, Qingdao 266003, China.
Hanyi CuiCollege of Food Science and Engineering, Ocean University of China, No.1299 Sansha Road, Qingdao 266003, China.
Jichao LiCollege of Food Science and Engineering, Ocean University of China, No.1299 Sansha Road, Qingdao 266003, China.
Haijin MouCollege of Food Science and Engineering, Ocean University of China, No.1299 Sansha Road, Qingdao 266003, China.
Dongyu LiCollege of Food Science and Engineering, Ocean University of China, No.1299 Sansha Road, Qingdao 266003, China.

Funding

China Postdoctoral Science Foundation 2021M701547National Natural Science Foundation of China 32202064National Natural Science Foundation of China 42106110Natural Science Foundation of Jiangxi Province 20224BAB215044Shandong Province Key R&D Program 2022TZXD005Shandong Province Marine Aquaculture Innovation and Entrepreneurship Community Project YZ2024002Youth Innovation Team Program of Universities in Shandong Province 2023KJ040
6 · The paper itself

Abstract

High viscosity of alginate means a relatively low substrate concentration, which limits the efficiency of hydrolysis, resulting in one of the main challenges for the large-scale production of alginate oligosaccharides (AOS). In this study, a pilot-scale degradation product (PSDP) of the recombinant enzyme 102C300C-Vgb was produced for the first time at a substrate concentration of up to 20% sodium alginate. The optimal conditions for SA digestion were: enzyme dosage of 25 U/g, enzymatic temperature of 45 °C, enzymatic pH of 7.0, and enzymatic time of 24 h. Under these conditions, the yield of enzymatic hydrolysis was consistently in the range of 69% to 70%. The average molecular weight (Mw) of PSDP was 1496.36 Da, mainly containing oligosaccharides with degrees of polymerization ranging from 2 to 4. The low-Mw PSDP was subsequently applied in the diet of sea cucumber

Indexed as

AlginatesStichopusAnimalsHydrogen-Ion ConcentrationHydrolysisMolecular WeightOligosaccharidesRecombinant ProteinsAlginatesOligosaccharidesRecombinant Proteinsalginate oligosaccharidesenzymolysisgut health

Identifiers

PMID41003308
PMCPMC12472054

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.