Evidence map›Paper›PMID 41002970›Full record

ArticleMetabolites2025

Aikaterina L Stefi, Maria Chalkiadaki, Emily Bashari, Konstantina Mitsigiorgi, Paweł Szczeblewski, Danae Papageorgiou, Dimitrios Gkikas, Dido Vassilacopoulou, Nikolaos S Christodoulakis, Maria Halabalaki

Abstract read
In one paragraph

Article in Metabolites, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Aikaterina L StefiSection of Botany, Department of Biology, Faculty of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece.ORCID 0000-0001-7395-7768
Maria ChalkiadakiDivision of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, 15784 Athens, Greece.ORCID 0009-0002-7199-8907
Emily BashariSection of Botany, Department of Biology, Faculty of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece.ORCID 0009-0005-0525-1735
Konstantina MitsigiorgiSection of Botany, Department of Biology, Faculty of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece.
Paweł SzczeblewskiDepartment of Pharmaceutical Technology and Biochemistry and BioTechMed Centre, Chemical Faculty, Gdansk University of Technology, 80-233 Gdansk, Poland.ORCID 0000-0001-9817-5631
Danae PapageorgiouSection of Botany, Department of Biology, Faculty of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece.
Dimitrios GkikasSection of Botany, Department of Biology, Faculty of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece.
Dido VassilacopoulouSection of Biochemistry and Molecular Biology, Department of Biology, Faculty of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece.
Nikolaos S ChristodoulakisSection of Botany, Department of Biology, Faculty of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece.ORCID 0000-0001-9292-876X
Maria HalabalakiDivision of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, 15784 Athens, Greece.ORCID 0000-0001-5620-0165

Funding

HORIZON-MSCA-2022-SE-01 101131346
6 · The paper itself

Abstract

BACKGROUND/

objectives

methodsSeedlings were cultivated under controlled conditions and subjected to either the control temperature (22 °C) or the heat stress temperature (35 °C) for one week. Morphological and anatomical traits were assessed, along with physiological parameters such as chlorophyll content, malondialdehyde (MDA), hydrogen peroxide (H

resultsHeat stress reduced dry mass and stomatal density but increased the diameter of the root transition zone, indicating anatomical adaptation. Leaves exhibited elevated oxidative stress markers and altered metabolite accumulation, while the roots showed a more integrated stress response. LC-HRMS/MS profiling revealed significant shifts in Cucurbitacin composition. Extracts from heat-stressed plants displayed stronger cytotoxicity, particularly toward DU-145 and SH-SY5Y cells, correlating with higher levels of glycosylated Cucurbitacins.

conclusions

Indexed as

cytotoxicDU-145Ecballium elateriumheat stressLC-HRMS/MSMCF-7 cellsSH-SY5Y cells

Identifiers

PMID41002970
PMCPMC12471846

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.