RNA Sequencing Identified Differentially Expressed Genes in the Mesocorticolimbic and Nigrostriatal Systems of Compulsive METH-Taking Rats.

Nasser Adjei, Bruce Ladenheim, Michael T McCoy, Vikrant Palande, Jean Lud Cadet, Atul P Daiwile

Abstract read

In one paragraph

Article in Cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Nasser AdjeiMolecular Neuropsychiatry Research Branch, NIDA Intramural Research Program, Baltimore, MD 21224, USA.

Bruce LadenheimMolecular Neuropsychiatry Research Branch, NIDA Intramural Research Program, Baltimore, MD 21224, USA.

Michael T McCoyMolecular Neuropsychiatry Research Branch, NIDA Intramural Research Program, Baltimore, MD 21224, USA.ORCID 0000-0001-7457-7660

Vikrant PalandeDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA.ORCID 0000-0002-3062-2105

Jean Lud CadetMolecular Neuropsychiatry Research Branch, NIDA Intramural Research Program, Baltimore, MD 21224, USA.ORCID 0000-0001-5635-3524

Atul P DaiwileMolecular Neuropsychiatry Research Branch, NIDA Intramural Research Program, Baltimore, MD 21224, USA.

Funding

NIH, NIDA-IRP DA000552

6 · The paper itself

Abstract

Methamphetamine (METH) is an extremely addictive drug which continues to cause significant harm to individuals and communities. In the present study we trained male rats to self-administer METH for 20 days, followed by 9 days of foot shock exposure. All rats escalated their METH intake during the first 20 days. The rats that continued to self-administer METH in the presence of aversive stimuli were termed shock-resistant (SR), while those that reduced their intake were shock-sensitive (SS). RNA sequencing showed numerous differentially expressed genes (DEGs) in the prefrontal cortex, nucleus accumbens, dorsal striatum, and midbrain. Ingenuity pathway analysis linked DEGs to addiction-related mechanisms. We identified shared genes with similar expression patterns across four brain regions (SR:

Indexed as

Compulsive BehaviorCorpus StriatumMethamphetamineSequence Analysis, RNAAnimalsGene Expression RegulationMaleNucleus AccumbensPrefrontal CortexRatsRats, Sprague-DawleyMethamphetaminedorsal striatumfoot-shocksmethamphetamine self-administrationmidbrainnucleus accumbensprefrontal cortexRNA sequencing

Identifiers

PMID41002437

DOI10.3390/cells14181472

PMCPMC12468296

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.