ArticleFrontiers in cellular and infection microbiology2025
Parasite-mediated alteration of behaviour and biomolecular dynamics in a mouse model.
Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- From parasite-induced immune activation to neuroinflammation and behavioral dysfunction: convergent mechanisms across protozoa and helminths: a review.Journal of neuroinflammation · 2026Review
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6 authors.
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Abstract
Introduction: Blastocystis is a highly prevalent gut parasite whose pathogenicity remains unclear. Both beneficial and detrimental effects have been observed as a result of Blastocystis infection, including altered gut microbiota, metabolism, and gastrointestinal health. The parasite expresses a modified tryptophanase enzyme known as BhTnaA, which has the unique ability to metabolize indole to tryptophan. Enterochromaffin cells in the gut produce serotonin from tryptophan. These cells are innervated by the vagus nerve, which serves an essential role in mediating bidirectional signaling between the gut and brain. Perturbed serotonin signaling has been associated with disorders linked to gut-brain axis dysfunction, such as IBS and some mood disorders. Our study shows that Blastocystis can use BhTnaA to influence serotonin synthesis by enterochromaffin cells Methods: We used RIN14B cells as an enterochromaffin cell model to determine whether BhTnaA upregulates serotonin synthesis and associated gene expression. Murine models colonized with multiple Blastocystis ST7 isolates were used to study altered serotonin metabolite levels in the gut. Analysis of mouse behavioral changes was done through the Light Box, Tail Suspension, and Open Field tests. Results: We demonstrated that the tryptophan produced by BhTnaA upregulates serotonin synthesis in EC cell models. In mice colonized with Blastocystis, increased tryptophan and serotonin levels were observed in the colon, a region of the gut inhabited by the parasites. Behavioral tests showed heightened anxiety in these mice, and a statistical correlation was identified between increases in the metabolites and observed anxiety behaviour. Discussion: Our study confirmed perturbation of gut tryptophan and serotonin levels by Blastocystis and showed a distinct correlation between this and increased anxiety in colonized mice. This provides a foundation for further investigation into the effects of these parasites on host physiology and the modulation of the gut-brain axis. LSID Identifiers:
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