Evidence map›Paper›PMID 41001049›Full record

ArticleFrontiers in microbiology2025

Identification of clinically relevant multi-drug resistant ESKAPEE isolates from hospital wastewater surveillance in Thailand.

Sidhartha Chaudhury, Wilawan Oransathit, Dutsadee Peerapongpaisarn, Wirote Oransathit, Chatchadaporn Thamnurak, Chantida Pradipol, Kirakarn Kirativanich, Sirigade Ruekit, Tanit Boonsiri, Yutthana Thanyathada and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Sidhartha ChaudhuryDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), Bangkok, Thailand.
Wilawan OransathitDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), Bangkok, Thailand.
Dutsadee PeerapongpaisarnDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), Bangkok, Thailand.
Wirote OransathitDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), Bangkok, Thailand.
Chatchadaporn ThamnurakDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), Bangkok, Thailand.
Chantida PradipolDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), Bangkok, Thailand.
Kirakarn KirativanichDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), Bangkok, Thailand.
Sirigade RuekitDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), Bangkok, Thailand.
Tanit BoonsiriDepartment of Microbiology, Phramongkutklao College of Medicine, Bangkok, Thailand.
Yutthana ThanyathadaClinical Microbiology Laboratory, Queen Sirikit Naval Hospital, Chonburi, Thailand.
Anjali P SapreMultidrug-Resistant Organism Repository and Surveillance Network (MRSN), Walter Reed Army Institute of Research, Silver Spring, Maryland, United States.
Katelyn V BartlettDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), Bangkok, Thailand.
Melissa J MartinMultidrug-Resistant Organism Repository and Surveillance Network (MRSN), Walter Reed Army Institute of Research, Silver Spring, Maryland, United States.
Paphavee LertsethtakarnDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), Bangkok, Thailand.
Pattaraporn VanachayangkulDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), Bangkok, Thailand.
Jeffrey R LivezeyDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), Bangkok, Thailand.
Daniel M BoudreauxDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), Bangkok, Thailand.
Nattaya RuamsapDepartment of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), Bangkok, Thailand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Wastewater surveillance has recently emerged as a promising method for AMR surveillance, but few studies have directly compared wastewater surveillance samples with clinical isolates and the clinical significance of wastewater surveillance for AMR bacteria is still unclear. Methods: We carried out monthly surveillance of hospital wastewater and surrounding community water at a tertiary hospital in Chonburi, Thailand from March to December 2024 and compared our findings with clinical isolates collected at the same hospital during the same period. For each wastewater sample, we isolated ESKAPEE pathogens, identified species by mass spectrometry, performed antimicrobial susceptibility testing (AST), followed by PCR testing of AMR genes and whole genome sequencing (WGS) on a subset of wastewater isolates and compared our results to clinical isolates. Results: We obtained 2,735 AMR isolates from untreated hospital wastewater, of which 1,550 were ESKAPEE pathogens including: Conclusion: Our results show that surveillance of untreated hospital wastewater is capable of identifying ESKAPEE that share similar drug resistance profiles, AMR genes, and clonal lineages found in the clinical isolates over the same time period. To our knowledge, this is one of the first studies to demonstrate a high level of genetic-relatedness between hospital wastewater and clinical isolates and demonstrate the clinical relevance of bacterial wastewater surveillance for MDR ESKAPEE pathogens.

Indexed as

antimicrobial resistancecarbapenemaseESBLESKAPEEMDRone healthThailandwastewater

Identifiers

PMID41001049
PMCPMC12457344

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.