Evidence map›Paper›PMID 41000917›Full record

ArticlebioRxiv : the preprint server for biology2025

Size-Modulated Mesoderm-Endoderm Divergence and Myocardial Cavitation in Micropatterned Cardioids.

Plansky Hoang, David W McKellar, Andrew Kowalczewski, Nhu Y Mai, Meng Chai, Xiaojun L Lian, Yi Zheng, Jeffrey Amack, Nathan Tucker, Iwijn De Vlaminck and 3 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Plansky HoangDepartment of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY, USA.
David W McKellarMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.ORCID 0000-0002-7752-078X
Andrew KowalczewskiDepartment of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY, USA.
Nhu Y MaiDepartment of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY, USA.
Meng ChaiDepartment of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY, USA.
Xiaojun L LianDepartment of Biomedical Engineering, Pennsylvania State University, University Park, PA, USA.
Yi ZhengDepartment of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY, USA.
Jeffrey AmackDepartment of Cell and Developmental Biology, Upstate Medical University, Syracuse, NY, USA.
Nathan TuckerDepartment of Pharmacology, Upstate Medical University, Syracuse, NY, USA.
Iwijn De VlaminckMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
Huaxiao YangDepartment of Biomedical Engineering, University of North Texas, Denton, TX.
Benjamin D CosgroveMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.ORCID 0000-0003-2164-350X
Zhen MaDepartment of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY, USA.ORCID 0000-0001-5228-105X

Funding

Establishing an In Vitro Embryotoxicity Risk Classification System Based on Human Cardiac Organoid ModelR01HD101130 · NICHD · SYRACUSE UNIVERSITY · PI MA, ZHEN · 2020 to 2025
$2.3M
Nanowired hypoimmunogenic hPSC cardiac organoids for heart repairR01HL175050 · NHLBI · CLEMSON UNIVERSITY · PI XIAOJUN LIAN, Ying Mei · 2024 to 2026
$2.0M
ModRNA-based Direct Programming of Universal Donor hiPSCs into Immune Evasive Beta CellsR56DK133147 · NIDDK · PENNSYLVANIA STATE UNIVERSITY, THE · PI LIAN, XIAOJUN · 2023 to 2024
$465k
NOTCH signaling on the underdeveloped cardiac vascularization of hypoplastic left heart syndrome in the hiPSC-derived vascularized cardiac organoidsR15HD108720 · NICHD · UNIVERSITY OF NORTH TEXAS · PI YANG, HUAXIAO · 2022 to 2022
$438k
NHLBI NIH HHS R01 HL175050NICHD NIH HHS R01 HD101130NICHD NIH HHS R15 HD108720NIDDK NIH HHS R56 DK133147
6 · The paper itself

Abstract

The human heart, originating from the splanchnic mesoderm, is the first functional organ to develop, co-evolving with the foregut endoderm through reciprocal signaling. Previously, cardioid models offered new insights on cardiovascular cell lineages and tissue morphogenesis during heart development, while mesoderm-endoderm crosstalk remain incompletely understood. Here, we integrated micropatterned cardioids, CRISPR-engineered reporter hiPSCs, deep-tissue imaging, and single-cell RNA sequencing (scRNA-seq) to explore synergistic mesoderm-endoderm co-development. scRNA-seq with PHATE trajectory mapping reconstructed lineage bifurcations of mesoderm-heart and endoderm-foregut lineages, identifying key cell types in cardiac and hepatic development. Ligand-receptor interaction analysis highlighted mesodermal cells enriched in non-canonical WNT, NRG, and TGF-β signaling, while endodermal cells exhibited VEGF and Hedgehog activity. We found that micropattern sizes influenced cellular composition, cardioid cavitation, contractile functions, and mesoderm-endoderm signaling crosstalk. The cardioids generated from 600 μm diameter circle patterns showed larger cavity formation resembling early heart chamber formation. Our findings establish micropatterned cardioids as a model for mesoderm-endoderm co-development, enhancing our understanding of heart-foregut synergy during early embryogenesis.

Indexed as

CardioidsHuman Induced Pluripotent Stem CellsMicropatterningSingle-Cell RNA sequencingSystems Biology

Identifiers

PMID41000917
PMCPMC12458260

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.