Evidence map›Paper›PMID 41000636›Full record

ArticlebioRxiv : the preprint server for biology2025

Patch-Clamp Single-Cell Proteomics in Acute Brain Slices: A Framework for Recording, Retrieval, and Interpretation.

Larry Rodriguez, Jolene Diedrich, Le Sun, Blake Tsu, Stefanie Kairs, Roman Vlkolinsky, Christopher A Barnes, Aline M A Martins, Marisa Roberto, John R Yates

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Larry RodriguezDepartment of Integrated Structural and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037.ORCID 0000-0002-9591-5005
Jolene DiedrichDepartment of Integrated Structural and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037.
Le SunDepartment of Integrated Structural and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037.
Blake TsuNGeneBioAI, Inc. San Diego, CA 92121.
Stefanie KairsNGeneBioAI, Inc. San Diego, CA 92121.
Roman VlkolinskyDepartment of Translational Medicine, The Scripps Research Institute, La Jolla, CA 92037.
Christopher A BarnesNGeneBioAI, Inc. San Diego, CA 92121.ORCID 0000-0003-2312-7082
Aline M A MartinsDepartment of Integrated Structural and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037.
Marisa RobertoDepartment of Translational Medicine, The Scripps Research Institute, La Jolla, CA 92037.
John R YatesDepartment of Integrated Structural and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037.ORCID 0000-0001-5267-1672

Funding

Viral Vector CoreP60AA006420 · NIAAA · SCRIPPS RESEARCH INSTITUTE, THE · PI AMANDA J ROBERTS · 2003 to 2026
$46.3M
Neurpsychopharmacology-Multidisciplinary TrainingT32AA007456 · NIAAA · SCRIPPS RESEARCH INSTITUTE, THE · PI MARISA ROBERTO · 1985 to 2026
$13.4M
Electrophysiology of alcohol in extended amygdelaU01AA013498 · NIAAA · SCRIPPS RESEARCH INSTITUTE, THE · PI MARISA ROBERTO · 2001 to 2026
$12.6M
Proteogenetics of Autism Spectrum DisordersR01MH100175 · NIMH · SCRIPPS RESEARCH INSTITUTE, THE · PI MUOTRI, ALYSSON R., YATES III, JOHN R · 2013 to 2025
$7.4M
SYSTEMIC NEUROPHARMACOLOGYP50AA006420 · NIAAA · SCRIPPS RESEARCH INSTITUTE · PI RIVIER, CATHERINE L · 1985 to 2002
$5.1M
Neuroplasticity of the Extended Amygdala CRF circuitry in alcohol dependenceR01AA021491 · NIAAA · SCRIPPS RESEARCH INSTITUTE, THE · PI ROBERTO, MARISA · 2013 to 2024
$3.9M
Gene-environment interaction: the brain CRF system in alcohol preferring msP ratsR37AA017447 · NIAAA · SCRIPPS RESEARCH INSTITUTE, THE · PI ROBERTO, MARISA · 2016 to 2025
$3.7M
Synaptic Mechanisms underlying sex-differences in alcohol use disorderR01AA029841 · NIAAA · SCRIPPS RESEARCH INSTITUTE, THE · PI MARISA ROBERTO · 2022 to 2026
$2.0M
Gene-environment interaction: the brain CRF system in alcohol preferring msP ratsR01AA017447 · NIAAA · SCRIPPS RESEARCH INSTITUTE, THE · PI ROBERTO, MARISA · 2009 to 2013
$1.8M
High Throughput Methods for Single Cell ProteomicsR21MH129776 · NIMH · SCRIPPS RESEARCH INSTITUTE, THE · PI YATES III, JOHN R · 2022 to 2023
$493k
NIAAA NIH HHS P50 AA006420NIAAA NIH HHS P60 AA006420NIAAA NIH HHS R01 AA017447NIAAA NIH HHS R01 AA021491NIAAA NIH HHS R01 AA029841NIAAA NIH HHS R37 AA017447NIAAA NIH HHS T32 AA007456NIAAA NIH HHS U01 AA013498NIMH NIH HHS R01 MH100175NIMH NIH HHS R21 MH129776
6 · The paper itself

Abstract

Single-cell proteomics (SCP) is a powerful method for interrogating the molecular composition of neurons, yet its application to acute brain slices has remained limited. Patch-clamp electrophysiology provides direct information on neuronal excitability, synaptic inputs, and ion channel function, making it a natural partner for SCP. However, combining these techniques introduces unique challenges: neurons must be both physiologically characterized and physically collected, and variability during retrieval from the brain slice can affect how faithfully proteomic measurements reflect

Indexed as

acute brain slicesG protein-coupled receptors (GPCRs)ion channelspatch-clamp electrophysiologysingle-cell proteomicssynaptic proteins

Identifiers

PMID41000636
PMCPMC12458218

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.