Evidence map›Paper›PMID 41000278›Full record

ArticleBreeding science2025

Development and interlaboratory validation of a cultivar-specific identification method for the table grape 'Shine Muscat' using loop-mediated isothermal amplification (LAMP).

Reona Takabatake, Yuki Monden, Akiko Shindo, Yasutaka Minegishi, Fumiya Taniguchi, Yu Hashimoto, Tomoyuki Takeuchi, Kazuto Takasaki, Sachiko Isobe

Abstract read
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In one paragraph

Article in Breeding science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Reona TakabatakeInstitute of Food Research, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Yuki MondenGraduate School of Environmental, Life, Natural Science, and Technology, Okayama University, 1-1-1 Tsushimanaka, Kitaku, Okayama, Okayama 700-8530, Japan.
Akiko ShindoGraduate School of Environmental, Life, Natural Science, and Technology, Okayama University, 1-1-1 Tsushimanaka, Kitaku, Okayama, Okayama 700-8530, Japan.
Yasutaka MinegishiNippon Gene Co., Ltd., 1-5, Kandanishiki-cho, Chiyoda-ku, Tokyo 101-0054, Japan.
Fumiya TaniguchiInstitute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki 305-8605, Japan.
Yu HashimotoInstitute of Food Research, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Tomoyuki TakeuchiFASMAC Co., Ltd., 3088 Okata, Atsugi, Kanagawa 243-0021, Japan.
Kazuto TakasakiFASMAC Co., Ltd., 3088 Okata, Atsugi, Kanagawa 243-0021, Japan.
Sachiko IsobeKazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

'Shine Muscat' is an elite table grape cultivar developed by the National Agriculture and Food Research Organization in Japan. Recently, the infringement of breeders' rights in various fruits has become a serious problem in Japan. In this study, a loop-mediated isothermal amplification (LAMP)-mediated cultivar identification method for 'Shine Muscat' was developed. We comprehensively analyzed retrotransposon insertion sites using 24 major grape cultivars and identified two insertions, VINE1-Cl160 and VINE1-Cl155, which are unique to 'Shine Muscat'. LAMP primers targeting VINE1-Cl160 and VINE1-Cl155 were designed, and specific amplifications were confirmed. We also designed a primer set to detect the grape endogenous reference sequence, UDP-glucose:flavonoid 3-

Indexed as

cultivar identificationDNA chromatographyLAMPretrotransposonShine Muscat

Identifiers

PMID41000278
PMCPMC12457787

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.