ReviewChemical & biomedical imaging2025
Advancing Single-Molecule Biophysics: Next-Generation Organic Fluorophores with Tailored Labeling Strategies.
Review in Chemical & biomedical imaging, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
7 citing papers in PubMed.
- Imaging from Macro to Nanoscale: Multimodal Advances in Chemical and Biomedical Imaging.Chemical & biomedical imaging · 2026Review
- Single Fluorogens and Orientation-Localization Microscopy for Quantifying Chemical and Biomolecular Dynamics at the Nanoscale.Accounts of chemical research · 2026Article
- Solvatochromic Study of 2‑(ACS omega · 2026Article
- Covalent ferrocene conjugation as an intramolecular strategy for photostability in fluorescein.Scientific reports · 2026Article
- A bright and regenerative array tag with fluorogenic ligands for long-term live-cell nanoscopy and single-molecule tracking.Nature communications · 2025Article
- Bridged-Bicyclic Fluorophores Push Photophysical Boundaries for Live-Cell Imaging.Chemical & biomedical imaging · 2025Article
- Advancing Multicolor Super-Resolution Volume Imaging: Illuminating Complex Cellular Dynamics.JACS Au · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent advancements in single-molecule biophysics have been driven by breakthroughs in advanced fluorescence microscopy techniques and the development of next-generation organic fluorophores. These cutting-edge fluorophores, coupled through tailored biolabeling strategies, offer single-molecule brightness, photostability, and phototunability (i.e., photoswitchable, photoactivatable), contributing to enhancing spatial and temporal imaging resolution for studying biomolecular interactions and dynamics at single-event precision. This review examines the progress made over the past decade in the development of next-generation fluorophores, along with their site-specific labeling methods for proteins, nucleic acids, and biomolecular complexes. It also explores their applications in single-molecule fluorescence-based dynamic structural biology and super-resolution microscopy imaging. Furthermore, it examines ongoing efforts to address challenges associated with fluorophore photostability, photobleaching, and the integration of advanced photophysical and photochemical functionalities. The integration of state-of-the-art fluorophores with advanced labeling strategies aim to deliver complementary correlative data, holding promise for revolutionizing single-molecule biophysics by pushing the boundaries of temporal and spatial imaging resolution to unprecedented limits.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.