ArticleBio-protocol2025
Detection of Autophagy in Human Peripheral Blood Mononuclear Cells Using Guava
Article in Bio-protocol, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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5 authors.
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Abstract
Autophagy plays a crucial role in cellular homeostasis and is responsible for removing and degrading damaged cytoplasmic cargo. This lysosome-mediated catabolic process removes defective organelles and misfolded proteins, and impaired autophagy has been directly linked to ageing and numerous diseases. This emphasises the importance of developing intervention methods to counteract this dysregulation. One promising intervention is thermal therapy, specifically hyperthermia, which is described in this protocol. In order to investigate this form of treatment, a rapid and reliable detection method is required to allow comparison of autophagy status under different conditions. While methods such as transmission electron microscopy (TEM) or western blotting can provide valuable structural analysis, they are often time-consuming and expensive, and are not suitable for small, round cells such as peripheral blood mononuclear cells (PBMCs). The method described in this protocol enables absolute quantification of PBMCs using the Guava
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