Evidence map›Paper›PMID 40999547›Full record

ArticleVeterinary research2025

Intranasal NS1-truncated live attenuated canine influenza vaccine confers superior protection compared to inactivated vaccine in beagles.

Jaehyun Hwang, Sun-Woo Yoon, Eulhae Ga, Jaeseok Choi, Suyun Moon, Eunseo Bae, Hyeongcheol Yun, Dohyeok Yu, Hye Kwon Kim, Jung-Ah Kang and 6 more

Abstract read
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Article in Veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Jaehyun Hwang *College of Veterinary Medicine, Chonnam National University, Gwangju, 61186, Republic of Korea.
Sun-Woo Yoon *Department of Biological Sciences and Biotechnology, Andong National University, Andong, 36729, Republic of Korea.
Eulhae GaCollege of Veterinary Medicine, Chonnam National University, Gwangju, 61186, Republic of Korea.
Jaeseok ChoiDental Research Institute, School of Dentistry, Seoul National University, Seoul, 03080, Republic of Korea.
Suyun MoonDepartment of Oral Microbiology and Immunology, School of Dentistry, Seoul National University, Seoul, 03080, Republic of Korea.
Eunseo BaeDepartment of Oral Microbiology and Immunology, School of Dentistry, Seoul National University, Seoul, 03080, Republic of Korea.
Hyeongcheol YunDepartment of Oral Microbiology and Immunology, School of Dentistry, Seoul National University, Seoul, 03080, Republic of Korea.
Dohyeok YuDepartment of Oral Microbiology and Immunology, School of Dentistry, Seoul National University, Seoul, 03080, Republic of Korea.
Hye Kwon KimDepartment of Biological Sciences and Biotechnology, College of National Sciences, Chungbuk National University, Cheongju, 28644, Republic of Korea.
Jung-Ah KangBionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Minjoo YeomDepartment of Virology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, Republic of Korea.
Jong-Woo LimDepartment of Virology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, Republic of Korea.
Dae Gwin JeongBionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Xing XieKey Laboratory for Veterinary Bio-Product Engineering, Ministry of Agriculture and Rural Affairs, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Daesub SongDepartment of Virology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, Republic of Korea. sds@snu.ac.kr.
Woonsung NaDental Research Institute, School of Dentistry, Seoul National University, Seoul, 03080, Republic of Korea. wsna1@snu.ac.kr.ORCID http://orcid.org/0000-0002-7254-5240

Funding

Ministry of Food and Drug Safety 22183MFDS446
6 · The paper itself

Abstract

Canine influenza virus (CIV) H3N2 continues to circulate among companion animals, posing a zoonotic risk due to its potential for cross-species transmission. However, currently available inactivated vaccines offer limited mucosal immunity and suboptimal protection. Here, we developed a novel intranasal live attenuated CIV H3N2 vaccine carrying a truncated nonstructural protein 1 (NS1) gene and evaluated its safety, immunogenicity, and protective efficacy in beagle dogs. The NS1-truncated LAIV was well-tolerated and induced robust mucosal and systemic immune responses, including high titers of virus-specific secretory IgA. Following challenge with virulent CIV H3N2 at 120 days post-vaccination, LAIV-immunized dogs exhibited complete clinical protection and minimal viral shedding, whereas dogs receiving the inactivated vaccine showed moderate disease signs. These findings demonstrate that the NS1-truncated LAIV confers superior protection compared to conventional vaccines and represents a promising next-generation platform for canine influenza control within a One Health framework.

Indexed as

Dog DiseasesInfluenza A Virus, H3N2 SubtypeInfluenza VaccinesOrthomyxoviridae InfectionsViral Nonstructural ProteinsAdministration, IntranasalAnimalsDogsFemaleMaleVaccines, AttenuatedVaccines, InactivatedInfluenza VaccinesINS1 protein, influenza virusVaccines, AttenuatedVaccines, InactivatedViral Nonstructural Proteinsbeagle dog modelCanine influenza virus (H3N2)intranasal vaccinelive attenuated influenza vaccine (LAIV)mucosal immunityNS1-truncated vaccinesterile immunity

Identifiers

PMID40999547
PMCPMC12465203

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.