Evidence map›Paper›PMID 40999528›Full record

ArticleVeterinary research2025

A novel function of RHOA as a host-dependent factor in Glaesserella parasuis infection of LLC-PK1 cells.

Huanhuan Zhou, Xuexue Chen, Xinqi Zeng, Shengsong Xie, Xiaoyu Zhang, Jiayi Zeng, Ke Xu, Bo Yu, Hailong Liu, Hongbo Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Huanhuan Zhou *Laboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.ORCID http://orcid.org/0000-0002-3205-1449
Xuexue Chen *Laboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
Xinqi ZengLaboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
Shengsong XieKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
Xiaoyu ZhangLaboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
Jiayi ZengLaboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
Ke XuLaboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
Bo YuLaboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
Hailong LiuKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, 430070, China. hailongliu@webmail.hzau.edu.cn.ORCID http://orcid.org/0000-0001-6663-563X
Hongbo ChenLaboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, 430023, China. chenhongbo@whpu.edu.cn.

Funding

Hubei Provincial Science and Technology Plan 2023BEB036National Natural Science Foundation of China No.32172712National Natural Science Foundation of China No.32302723Wuhan Science and Technology Project No. 2022021302024853
6 · The paper itself

Abstract

Glaesserella parasuis (G. parasuis), a lethal pathogen causing Glässer's disease, poses severe threats to global swine health. While the small GTPase Ras homolog gene family member A (RHOA) is implicated in viral pathogenesis, its role in bacterial infections remains unexplored. Here, we established an in vitro infection model using porcine LLC-PK1 cells and demonstrated that G. parasuis induces adhesion and pseudopodia-mediated invasion, resulting in approximately 99% cell death within 120 h post-infection. Crucially, RHOA expression was upregulated during infection, and RHOA knockout reduced bacterial adhesion and invasion, rescuing cell viability to 77.30%. Transcriptomic profiling of RHOA-knockout cells revealed 1797 differentially expressed genes, revealing indirect effects on cytoskeleton remodeling (ACTG1/MYL7/MYL9 downregulation) and tight junction stabilization (CDH1/CLDN1/CDH5 upregulation). This establishes RHOA as a key host factor facilitating G. parasuis infection, providing targets for disease control.

Indexed as

Haemophilus InfectionsHaemophilus parasuisrhoA GTP-Binding ProteinSwine DiseasesAnimalsLLC-PK1 CellsSwinerhoA GTP-Binding ProteinadhesionGlaesserella parasuisinvasionpigRHOA

Identifiers

PMID40999528
PMCPMC12465878

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.