Evidence map›Paper›PMID 40999471›Full record

ArticleStem cell research & therapy2025

Optimizing the in vitro neuronal microenvironment to mitigate phototoxicity in live-cell imaging.

Cassandra R Hoffmann, Simon Maksour, Jordan E Clarke, Maciej Daniszewski, Fiona J Houghton, Jingqi Wang, Alice Pébay, Paul A Gleeson, Mirella Dottori, Ellie Cho and 2 more

Abstract read
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In one paragraph

Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Cassandra R HoffmannDepartment of Psychiatry, The University of Melbourne, Parkville, Australia. crho@student.unimelb.edu.au.ORCID http://orcid.org/0000-0003-1310-1794
Simon MaksourSchool of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, Australia.
Jordan E ClarkeDepartment of Anatomy and Physiology, The University of Melbourne, Parkville, Australia.
Maciej DaniszewskiDepartment of Anatomy and Physiology, The University of Melbourne, Parkville, Australia.
Fiona J HoughtonDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Australia.
Jingqi WangDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Australia.
Alice PébayDepartment of Anatomy and Physiology, The University of Melbourne, Parkville, Australia.
Paul A GleesonDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Australia.
Mirella DottoriSchool of Medical, Indigenous and Health Sciences and Molecular Horizons, University of Wollongong, Wollongong, Australia.
Ellie ChoBiological Optical Microscopy Platform, University of Melbourne, Parkville, Australia.
Andrew ZaleskyDepartment of Psychiatry, The University of Melbourne, Parkville, Australia.
Maria A Di BiaseDepartment of Psychiatry, The University of Melbourne, Parkville, Australia.

Funding

National Health and Medical Research Council 1175754National Health and Medical Research Council 2011592
6 · The paper itself

Abstract

Long-term imaging formats are ideal for capturing dynamic neuronal network formation in vitro, yet fluorescent techniques are often constrained by the impact of phototoxicity on cell survival. Here we present a live-imaging protocol that was optimised via quantitative analysis of 3 target culturing conditions on neuromorphological health: extracellular matrix (human- versus murine-derived laminin), culture media (Neurobasal™ versus Brainphys™ Imaging media), and seeding density (1 × 10

Indexed as

Cellular MicroenvironmentNeuronsAnimalsCell DifferentiationCell SurvivalHuman Embryonic Stem CellsHumansLamininMiceLaminin

Identifiers

PMID40999471
PMCPMC12465337

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.