Evidence map›Paper›PMID 40999254›Full record

ArticleWorld journal of microbiology & biotechnology2025

Glycolipopeptide biosurfactants from Serratia marcescens exhibit broad-spectrum antimicrobial activity for sustainable biocontrol of multidrug-resistant pathogens.

Amir Mahgoub Awadelkareem, Arif Jamal Siddiqui, Mitesh Patel, Syed Amir Ashraf, Insaf Bahrini, Yazeed Albulaihed, Mohammad Saquib Ashraf, Shahida Banu Shamsuddeen, Mohd Adnan

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Article in World journal of microbiology & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Amir Mahgoub AwadelkareemDepartment of Clinical Nutrition, College of Applied Medical Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia.
Arif Jamal SiddiquiDepartment of Biology, College of Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia.
Mitesh PatelDepartment of Computer Science and Bioscience, Faculty of Engineering and Technology, Marwadi University, Rajkot, 360003, Gujarat, India.
Syed Amir AshrafDepartment of Clinical Nutrition, College of Applied Medical Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia.
Insaf BahriniDepartment of Biology, College of Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia.
Yazeed AlbulaihedDepartment of Biology, College of Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia.
Mohammad Saquib AshrafDepartment of Medical Laboratory Science, College of Pharmacy, Nursing and Medical Science, Riyadh ELM University, P.O. Box 12734, Riyadh, Saudi Arabia.
Shahida Banu ShamsuddeenDepartment of Clinical Nutrition, College of Applied Medical Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia.
Mohd AdnanDepartment of Biology, College of Science, University of Ha'il, P.O. Box 2440, Ha'il, Saudi Arabia. mo.adnan@uoh.edu.sa.

Funding

University of Hail RG-23208
6 · The paper itself

Abstract

The rising prevalence of multidrug-resistant (MDR) pathogens across clinical, agricultural, and environmental settings underscores the urgent need for alternative antimicrobial agents. In this study, biosurfactants produced by Serratia marcescens were extracted, isolated, and characterized to assess their broad-spectrum antimicrobial potential. The S. marcescens-derived biosurfactant (SMBS) demonstrated strong surface activity, confirmed through oil spreading (19.4 mm), drop collapse, and a high emulsification index (%E24 of 74.8% with olive oil). Structural characterization using HPTLC, FTIR, and GC-MS suggested that SMBS is a complex glycolipopeptide comprising lipid, peptide, and carbohydrate moieties. Functionally, SMBS exhibited considerable antibacterial activity with inhibition zones of 12 mm, 11 mm, and 14 mm against Acinetobacter pittii, Proteus vulgaris, and Staphylococcus aureus, respectively. Antifungal assays revealed notable inhibition of Aspergillus niger (2 mm) Candida albicans (6 mm), Fusarium proliferatum (16 mm), and Penicillium purpurogenum (20 mm). While SMBS showed significant activity, it was comparatively less effective than streptomycin in most cases. These findings highlight the potential of SMBS as a sustainable, multifunctional biocontrol agent suitable for use in healthcare, agriculture, and environmental management. Its broad-spectrum efficacy, structural complexity, and biodegradability offer an attractive alternative to conventional antimicrobials. However, challenges such as large-scale purification, production cost, and the need for detailed toxicity and mechanism-of-action studies remain. Addressing these aspects will be essential for advancing SMBS toward clinical and commercial applications.

Indexed as

Anti-Infective AgentsBiological Control AgentsLipopeptidesSerratia marcescensSurface-Active AgentsAnti-Bacterial AgentsAntifungal AgentsBacteriaBiosurfactantsDrug Resistance, Multiple, BacterialFungiMicrobial Sensitivity TestsAnti-Bacterial AgentsAntifungal AgentsAnti-Infective AgentsBiological Control AgentsLipopeptidesSurface-Active AgentsAntibacterialAntifungalBiocontrol agentBiosurfactantGlycolipopeptideMultidrug-resistant pathogensSerratia marcescens

Identifiers

PMID40999254

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.