Incorporation of transmembrane protein antigens into phospholipid bilayers supported on silica microbeads using membrane fusion with budded virions of recombinant baculovirus.

Kohei Nakanishi, Seiwa Nishio, Yushi Isozaki, Masahiro Tomita, Kanta Tsumoto

Abstract read

In one paragraph

Article in Biotechnology letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Kohei NakanishiDivision of Chemistry for Materials, Graduate School of Engineering, Mie University, Tsu, Mie, 514-8507, Japan. k.nakanishi2018@gmail.com.

Seiwa NishioDivision of Chemistry for Materials, Graduate School of Engineering, Mie University, Tsu, Mie, 514-8507, Japan.

Yushi IsozakiDivision of Chemistry for Materials, Graduate School of Engineering, Mie University, Tsu, Mie, 514-8507, Japan.

Masahiro TomitaDivision of Chemistry for Materials, Graduate School of Engineering, Mie University, Tsu, Mie, 514-8507, Japan.

Kanta TsumotoDivision of Chemistry for Materials, Graduate School of Engineering, Mie University, Tsu, Mie, 514-8507, Japan. tsumoto@chem.mie-u.ac.jp.ORCID http://orcid.org/0000-0002-7882-3635

Funding

Japan Society for the Promotion of Science JP15H03541Japan Society for the Promotion of Science JP16K07293Japan Society for the Promotion of Science JP17H03468Japan Society for the Promotion of Science JP19K06540Japan Society for the Promotion of Science JP22H01920Japan Society for the Promotion of Science JP23K23188

6 · The paper itself

Abstract

For the reconstitution of artificial cell membranes, silica microbeads are often selected as scaffolds to reinforce fragile lipid membranes; these bilayer-covered beads are often called spherical supported lipid bilayer membranes (SS-BLMs). SS-BLMs were made through the adsorption of small unilamellar vesicles (SUVs) preformed from a mixture of phosphatidylcholine (PC) and phosphatidylserine (PS). Fluorescence microscopic observation showed that budded viruses (BVs) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) labeled with octadecyl rhodamine B chloride (R18) could fuse and be optimally distributed homogeneously over the SS-BLMs when using a combination of PC and PS. We also demonstrated that when BVs expressing the recombinant transmembrane protein, G-protein coupled receptor (GPCR), like β2 adrenergic receptor and corticotropin-releasing hormone receptor 1, the protein could be specifically visualized on SS-BLMs by immunofluorescence microscopy. This indicates that recombinant proteins were incorporated into the spherical supported lipid bilayers through virion-lipid bilayer membrane fusion.

Indexed as

Membrane LipidsMembrane ProteinsMembranes, ArtificialMicrospheresRecombinant ProteinsSilicon DioxideAdsorptionAnimalsNucleopolyhedrovirusesProteolipidsSf9 CellsSpodopteraMembrane LipidsMembrane ProteinsMembranes, ArtificialProteolipidsproteoliposomesRecombinant ProteinsSilicon DioxideAutographa californica multiple nucleopolyhedrovirusBaculovirusMembrane fusionSilica beadsSmall unilamellar vesiclesSpherical-supported lipid bilayer membranes

Identifiers

PMID40999245

DOI10.1128/JVI.02490-07

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.