ArticleThe Journal of nutrition2025
The Amount of Dietary Methionine Required to Maximize Synthesis of Transmethylated Products Is Higher than That Needed for Protein Synthesis in Neonatal Piglets.
Article in The Journal of nutrition, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMethionine is required for synthesizing protein and other critical metabolites, acting as a precursor for >50 transmethylation reactions to produce metabolites such as creatine, phosphatidylcholine (PC), and methylated DNA. In neonatal piglets, because only one-third of dietary methionine is incorporated into protein compared with two-thirds for methylated products, both protein and nonprotein demands need to be considered when determining the methionine requirement.
objectivesOur major objective was to quantify how much dietary methionine is required to maximize protein synthesis and key transmethylated products (creatine, PC, and methylated DNA).
methodsTwenty surgically altered Yucatan miniature piglets (8 ± 2 d) were fed complete diets for 5 d and then randomly assigned to 20 test diets with methionine intakes ranging from 20% to 220% of requirement [0.32-3.55 g methionine/100 g total amino acids (AAs)]. Stable isotopes of phenylalanine and tyrosine were administered to determine whole-body protein synthesis. [
resultsBreakpoint analysis indicated a whole-body requirement of 1.73 ± 0.27 g methionine/100 g total AAs. [
conclusionsThese data suggest that protein synthesis should not be used to determine whole-body requirements because more methionine is required to meet nonprotein demands for methionine.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.