Evidence map›Paper›PMID 40997799›Full record

ArticleDevelopmental cell2026

Maternal CENP-C restores centromere symmetry in mammalian zygotes to ensure proper chromosome segregation.

Catherine A Tower, Gabriel Manske, Emily L Ferrell, Dilara N Anbarci, Kelsey Jorgensen, Binbin Ma, Mansour Aboelenain, Rajesh Ranjan, Saikat Chakraborty, Lindsay Moritz and 9 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental cell, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

19 authors.

Catherine A TowerDepartment of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA.
Gabriel ManskeDepartment of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA; Cellular and Molecular Biology Graduate Program, University of Michigan, Ann Arbor, MI 48109, USA.
Emily L FerrellDepartment of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI 48109, USA.
Dilara N AnbarciDepartment of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA.
Kelsey JorgensenDepartment of Anthropology, University of Kansas, Lawrence, KS 66044, USA.
Binbin MaHoward Hughes Medical Institute, Department of Biology, John Hopkins University, Baltimore, MD 21218, USA.
Mansour AboelenainDepartment of Genetics, Rutgers University, Piscataway, NJ 08854, USA; Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, UK; Department of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Rajesh RanjanHoward Hughes Medical Institute, Department of Biology, John Hopkins University, Baltimore, MD 21218, USA.
Saikat ChakrabortyDepartment of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA.
Lindsay MoritzDepartment of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA.
Arunika DasDepartment of Biomedical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY 14853, USA.
Michele BoianiMax Planck Institute for Molecular Biomedicine, Department of Cell & Tissue Dynamics, Muenster 48149, Germany.
Ben E BlackDepartment of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA.
Shawn ChavezDivision of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR 97006, USA; Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR 97239, USA; Department of Obstetrics and Gynecology, Oregon Health and Science University, Portland, OR 97239, USA; Department of Biomedical Engineering, Oregon Health and Science University, Portland, OR 97239, USA.
Erica E MarshDepartment of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI 48109, USA.
Ariella ShikanovCellular and Molecular Biology Graduate Program, University of Michigan, Ann Arbor, MI 48109, USA; Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Karen SchindlerDepartment of Genetics, Rutgers University, Piscataway, NJ 08854, USA.
Xin ChenHoward Hughes Medical Institute, Department of Biology, John Hopkins University, Baltimore, MD 21218, USA.
Saher Sue HammoudDepartment of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA; Cellular and Molecular Biology Graduate Program, University of Michigan, Ann Arbor, MI 48109, USA; Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Urology, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: hammou@umich.edu.

Funding

XenograftP30CA046592 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Eric R. Fearon · 1988 to 2026
$178.2M
PREDOCTORAL TRAINING IN GENETICST32GM007544 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MORAN, JOHN V. · 1985 to 2022
$11.3M
Cellular and Molecular Biology at MichiganT32GM145470 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI John Chadwick Brenner · 2022 to 2026
$4.1M
Study epigenetic inheritance during development, homeostasis and regenerationR35GM127075 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Xin Chen · 2018 to 2026
$3.2M
Age and molecular mechanisms contributing to aneuploidy in oocytesR01HD058730 · NICHD · UNIVERSITY OF PENNSYLVANIA · PI BLACK, BEN E., LAMPSON, MICHAEL · 2009 to 2020
$3.2M
Signaling Mechanisms that Control Chromosome Segregation during Female Meiosis (Equipment Administrative Supplement)R35GM136340 · NIGMS · RUTGERS, THE STATE UNIV OF N.J. · PI Karen A Schindler · 2020 to 2026
$3.1M
The role of ZCWPW1 in meiosisR01GM148028 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Francesca Cole, Saher Sue Hammoud · 2023 to 2026
$2.4M
Contributions of Sperm Chromatin to development: A Myth or Reality?DP2HD091949 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HAMMOUD, SAHER SUE · 2016 to 2016
$2.3M
Mechanisms of sperm chromatin remodeling in vivo and in vitroR01HD113274 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Saher Sue Hammoud · 2023 to 2026
$2.0M
The mechanisms of MEIG1 complex in mammalian spermiogenesis and fertilizationR01HD114311 · NICHD · WAYNE STATE UNIVERSITY · PI Saher Sue Hammoud, Christopher V Kelly · 2024 to 2026
$1.9M
Sperm Chromatin: Implications on organismal development and fertilityR01HD104680 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HAMMOUD, SAHER SUE · 2021 to 2024
$1.8M
Comprehensive mapping of mouse testis cell types and spermatogenic stages by single-cell RNA sequencingR21HD090371 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HAMMOUD, SAHER SUE · 2018 to 2019
$444k
NCI NIH HHS P30 CA046592NICHD NIH HHS DP2 HD091949NICHD NIH HHS F31 HD100124NICHD NIH HHS F31 HD117648NICHD NIH HHS R01 HD058730NICHD NIH HHS R01 HD104680NICHD NIH HHS R01 HD113274NICHD NIH HHS R01 HD114311NICHD NIH HHS R21 HD090371NICHD NIH HHS R21 HD113812NIGMS NIH HHS R01 GM148028NIGMS NIH HHS R35 GM127075NIGMS NIH HHS R35 GM136340NIGMS NIH HHS T32 GM007544NIGMS NIH HHS T32 GM145470
6 · The paper itself

Abstract

Across metazoan species, the centromere-specific histone variant CENP-A is essential for accurate chromosome segregation, yet its regulation during the mammalian parental-to-zygote transition is poorly understood. To address this, we generated a CENP-A-mScarlet mouse model that revealed sex-specific dynamics: mature sperm retain 10% of the CENP-A levels present in MII oocytes. However, this difference is resolved in zygotes prior to the first mitosis, using maternally inherited cytoplasmic CENP-A. Notably, the increase in CENP-A at paternal centromeres is independent of sensing CENP-A asymmetry or the presence of maternal chromosomes. Instead, CENP-A equalization relies on the asymmetric recruitment of maternal CENP-C to paternal centromeres. Depletion of maternal CENP-A decreases total CENP-A in both pronuclei without disrupting equalization. In contrast, reducing maternal CENP-C or disruption of its dimerization function impairs CENP-A equalization and chromosome segregation. Therefore, maternal CENP-C acts as a key epigenetic regulator that resets centromeric symmetry at fertilization to preserve genome integrity.

Indexed as

CentromereChromosomal Proteins, Non-HistoneChromosome SegregationZygoteAnimalsCentromere Protein AFemaleMaleMiceMitosisOocytesCenpa protein, mouseCentromere Protein Acentromere protein CChromosomal Proteins, Non-HistoneCENP-ACENP-CcentromereepigeneticsintergenerationalMIS18BP1mouseoocytespermzygote

Identifiers

PMID40997799
PMCPMC12479092

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.