Evidence map›Paper›PMID 40996809›Full record

ArticleeLife2025

The effects of 17α-estradiol treatment on endocrine system revealed by single-nucleus transcriptomic sequencing of hypothalamus.

Lei Li, Guanghao Wu, Xiaolei Xu, Junling Yang, Lirong Yi, Ziqing Yang, Zheng Mo, Li Xing, Ying Shan, Zhuo Yu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eLife, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Lei Li *Department of Obstetrics and Gynecology, National Clinical Research Center for Obstetric and Gynecologic Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Guanghao Wu *School of Medical Technology，Beijing Institute of Technology, Beijing, China.
Xiaolei Xu *Department of Medical Oncology，Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China.
Junling YangDepartment of Cancer Biology and Pharmacology, University of Illinois College of Medicine, Peoria, United States.
Lirong YiInstitute of Reproductive Medicine, Medical School of Nantong University, Nantong, China.
Ziqing YangSchool of Basic Medical Sciences, Shandong University, Jinan, China.
Zheng MoDepartment of Medical Oncology，Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China.
Li XingDepartment of Medical Oncology，Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China.
Ying ShanDepartment of Obstetrics and Gynecology, National Clinical Research Center for Obstetric and Gynecologic Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Zhuo YuDepartment of Medical Oncology，Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China.
Yinchuan LiInstitute of Reproductive Medicine, Medical School of Nantong University, Nantong, China.ORCID https://orcid.org/0000-0002-8450-6126

Funding

National High Level Hospital Clinical Research Funding 2022-PUMCH-A-231National Natural Science Foundation of China 31271448National Natural Science Foundation of China 82171621National Natural Science Foundation of China 82172566
6 · The paper itself

Abstract

This study investigated 17α-estradiol's effects on aged hypothalamic physiological activity via long-term administration. Single-nucleus transcriptomic sequencing (snRNA-seq) was performed on pooled hypothalami from each group: aged male Norway brown rats treated with 17α-estradiol (O.T), aged controls (O), and young controls (Y). Supervised clustering of neurons (based on neuropeptides/receptors) evaluated subtype responses to aging and 17α-estradiol. Aging-induced elevation of neuronal cellular metabolism, stress, and reduced synapse formation-related pathways were significantly attenuated by 17α-estradiol. Neuron population analysis showed that subtypes regulating food intake, reproduction, blood pressure, stress response, and electrolyte balance were sensitive to 17α-estradiol. 17α-estradiol increased serum oxytocin (Oxt) and hypothalamic-pituitary-gonadal (HPG) axis activity (elevated plasma Gnrh, total testosterone; reduced estradiol). Gnrh1 upregulation mediated its effects on energy homeostasis, neural synapse, and stress response. Notably,

Indexed as

Endocrine SystemEstradiolHypothalamusTranscriptomeAgingAnimalsGene Expression ProfilingMaleNeuronsRatsEstradiol17α-estradiolagingcell biologycrhCrhgnrhGnrhhypothalamusneuroscienceoxtOxtsnrna-seqsnRNA-seq

Identifiers

PMID40996809
PMCPMC12463393

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.