ArticleBulletin of experimental biology and medicine2025
Transformed and Tumor Cells Spontaneously Produce DNA-Containing Extracellular Structures In Vitro.
Article in Bulletin of experimental biology and medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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6 authors.
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Abstract
The parameters of DNA-containing extracellular structures formed by immortalized fibroblasts and HCT116 adenocarcinoma cells during cultivation were studied. Fluorescence microscopy with SYBR Green dye was used to visualize DNA-containing extracellular structures. In contrast to intact neutrophils, immortalized fibroblasts and adenocarcinoma cells spontaneously formed DNA-containing extracellular structures during incubation. The process begins with the release of a single DNA-containing strand from the cell immediately after the cells are fixed to the substrate (1 h) and ends after 3 h with the formation of DNA-containing extracellular structures in the form of bundles of parallel fibers. These findings indicate active nature of the secretion of DNA-containing structures by immortalized fibroblasts and HCT116 adenocarcinoma cells.
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