ArticleCurrent protocols2025
Optimization of the Blocking and Signal Preservation Protocol in High-Parameter Flow Cytometry.
Article in Current protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Optimization of the Blocking and Signal Preservation Protocol in High-Parameter Flow Cytometry.Current protocols · 2025Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
High quality input data is the key to successful interpretation of any scientific assay. In flow cytometry, fluorescently-conjugated antibodies allow us to simultaneously measure an incredible range of protein-based targets on single cells with a high degree of specificity. Limiting the quality of data generated, however, is the non-specific interaction that can occur between antibodies and off-target binders. Judicious use of blocking reagents can improve the specificity of the staining by reducing this non-specific binding to cells, improving the sensitivity of the assay to detect the authentic signal above assay noise. Additional beneficial effects include preventing interactions between dyes and even limiting the degradation of dyes, improving data quality. In this article, we provide a workflow for minimizing these unwanted effects, increasing specificity and sensitivity in highly multiplex flow cytometry. © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Surface staining Basic Protocol 2: Intracellular staining Basic Protocol 3: Intracellular cytokine staining.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.