Evidence map›Paper›PMID 40996284›Full record

ArticleJournal of microbiology & biology education2025

Using CRISPRi in

Tammy J Bullwinkle

Abstract read
In one paragraph

Article in Journal of microbiology & biology education, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Tammy J BullwinkleDepartment of Microbiology, The Ohio State University, Columbus, Ohio, USA.ORCID 0009-0003-9683-0999

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A CRISPR interference (CRISPRi) exercise was developed for an upper-level molecular microbiology laboratory to reinforce student skills in experimental design and controls. This CRISPRi knockdown method is a variation of the commonly used

Indexed as

bacterial gene expressionCRISPRCRISPRiexperimental controlsPCRprimer design

Identifiers

PMID40996284
PMCPMC12687632

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.