ArticleJournal of virology2025
Discontinuous template switching generates coronavirus subgenomic RNAs from the 3' viral genome end by 5' to 3' transcription.
Article in Journal of virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Extra gene coding capacity of SARS-CoV-2 provides a virus engineering platform for in vitro and in vivo applications.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A stable subgenomic reporter coronavirus enables transcriptional profiling of bystander cells.The Journal of general virology · 2026Article
- RNA Folding Energy of Long-Range Genomic Interactions Regulates Discontinuous Transcription in SARS-CoV-2.Viruses · 2026Article
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Authors and funding
6 authors.
Funding
Abstract
In coronavirus (CoV)-infected cells, several structural and accessory proteins are synthesized from subgenome RNAs (sgRNA) containing a common genomic 5'-leader followed by a given open reading frame (ORF). We report that the abundance of these sgRNAs varies with distance from the 3'-end of the genome. Thus, there are more sgRNAs encoding nucleocapsid (N) than spike (S), presumably the results from discontinuous 5'-3' transcription template switch mediated by the viral replication and transcription complex (RTC). We optimized the circular polymerase extension reaction (CPER) methodology to generate infectious double-stranded circular cDNA (ds-circDNA) containing the mNeonGreen (NG) reporter in accessory ORFs of human CoVs OC43 and SARS-CoV-2. In each CoV, we found that levels of sgRNAs and NG expression increased with 3' proximal genomic NG location. By reinfection of HCT-8 cells with the same MOI 0.01, however, we found that the slow-growing OC43 NG-ns2 virions exhibited equal infectivity and productivity as the fast-growing OC43 NG-ns12.9 virions. Introduction of point-mutations into the mapped TRS
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