ArticleMethodsX2025
Comparative analysis of phage cocktail activity against uropathogenic
Article in MethodsX, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spot overlay assays are a common method for measuring phage activity against target microorganisms and determining % host range activity. Dilutions of phage demonstrate activity by creating a zone of clearing indicative of bacterial killing; however, this yields only semi-quantitative results for each target strain tested. In addition, zones can be completely clear or incomplete cloudy zones of varying intensity. These factors make it difficult to compare the overall strength of activity between monophages or phage cocktails when numerous bacterial strains are involved. Here we demonstrate a method to analyze spot overlay results to provide insight beyond just host range activity; three phage cocktails with host range activity of 92-96 % had different overall activities. While the spot overlay is a qualitative assay, consolidation of activity results can derive semi-quantitative information. This method converts activity of phage dilutions from a spot overlay assay into an overall activity of cocktails for comparison. Our method:•Uses standard laboratory protocol for analysis•Expands information beyond host range activity•Consolidates results of all strains tested to assess overall cocktail strength of activity for comparison.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.