ArticleEnvironmental DNA (Hoboken, N.J.)
Persistent gaps and errors in reference databases impede ecologically meaningful taxonomy assignments in 18S rRNA studies: a case study of terrestrial and marine nematodes.
Article in Environmental DNA (Hoboken, N.J.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed.
- Location and habitat specificity structure benthic microbial assemblages in a temperate seagrass ecosystem.mSphere · 2026Article
- Biogeographic and Habitat-Associated Variation in the Gut Microbiota of the Stingless BeeInsects · 2026Article
- Environmental gradients and habitat specificity structure benthic microbial assemblages in a temperate seagrass ecosystem.bioRxiv : the preprint server for biology · 2026Article
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Abstract
In metabarcoding studies, Linnaean taxonomy assignments of Operational Taxonomic Units (OTUs) or Amplicon Sequence Variants (ASVs) underpin many downstream bioinformatics analyses and ecological interpretations of environmental DNA (eDNA) datasets. However, public molecular databases (i.e., SILVA, EUKARYOME, BOLD) for most microbial metazoan phyla (nematodes, tardigrades, kinorhynchs, etc.) are sparsely populated, negatively impacting our ability to assign ecologically meaningful taxonomy to these understudied groups. Additionally, the choice of bioinformatics parameters and computational algorithms can further impact the accuracy of eDNA taxonomy assignments. Here, we use two
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