Evidence map›Paper›PMID 40993306›Full record

ArticleMikrochimica acta2025

An automated portable LAMP-based centrifugal microfluidic system for nucleic acid detection of multiple pathogens in feline upper respiratory disease.

Wangyu Bi, Fei Wen, Shanglin Cai, Yucui Li, Lei Zhang, Liang Bai, Jie Wang, Cencan Xing, Lei Zhang, Hongwu Du and 1 more

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Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Wangyu Bi *School of Chemistry and Biological Engineering, University of Science and Technology, Beijing, Beijing, 100083, China.
Fei Wen *Beijing Taihao Biotechnology Co., Ltd, Beijing, 102200, China.
Shanglin CaiSchool of Chemistry and Biological Engineering, University of Science and Technology, Beijing, Beijing, 100083, China.
Yucui LiBeijing Taihao Biotechnology Co., Ltd, Beijing, 102200, China.
Lei ZhangBeijing Taihao Biotechnology Co., Ltd, Beijing, 102200, China.
Liang BaiBeijing Taihao Biotechnology Co., Ltd, Beijing, 102200, China.
Jie WangSchool of Chemistry and Biological Engineering, University of Science and Technology, Beijing, Beijing, 100083, China.
Cencan XingDaxing Research Institute, University of Science and Technology, Beijing, Beijing, 100083, China.
Lei ZhangBeijing Quanxinquanyi Animal Clinic, Beijing, 100012, China. zhanglei861225@126.com.
Hongwu DuSchool of Chemistry and Biological Engineering, University of Science and Technology, Beijing, Beijing, 100083, China. hongwudu@ustb.edu.cn.
Lei WangDaxing Research Institute, University of Science and Technology, Beijing, Beijing, 100083, China. leiwang@ustb.edu.cn.

Funding

National Key Research and Development Program of China 2022YFC2603604
6 · The paper itself

Abstract

A portable centrifugal microfluidic nucleic acid analyzer tailored for point-of-care diagnostics was developed. The system consists of a main unit and a sector-shaped microfluidic chip driven by centrifugal force. By incorporating branched microchannels into the chip design, the system enables simultaneous detection of multiple targets from a single sample. The sample flows sequentially through the sample reservoir, dissolution chamber, and metering structure before reaching the reaction chamber. Under the presence of specific primers, the analyzer performs loop-mediated isothermal amplification (LAMP) by heating the chip. Fluorescence signals are captured by the optical detection module and displayed in real-time. We designed highly specific primers targeting six common feline upper respiratory disease (FURD) pathogens and extensively validated the device's specificity and sensitivity. The system demonstrated a low detection limit (10-100 copies/μL) and successfully identified 72 clinically positive samples, achieving 100% agreement with conventional PCR. Weighing only 5 kg, the analyzer can accurately detect and identify six FURD-related pathogens from a single sample within 30 min. This method enables a shift from single-target to multitarget detection and promotes the broader adoption of decentralized testing. It offers an efficient and convenient solution for rapid and accurate pathogen detection, particularly in pet hospitals and veterinary clinical diagnostics.

Indexed as

Cat DiseasesLab-On-A-Chip DevicesMicrofluidic Analytical TechniquesMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesRespiratory Tract InfectionsAnimalsCatsCentrifugationLimit of DetectionFeline upper respiratory diseaseMicrofluidic deviceNucleic acid detectionPoint-of-care testing

Identifiers

PMID40993306

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.