ReviewNature metabolism2025
A consensus guide to preclinical indirect calorimetry experiments.
Abstract readReviewConsensus Statement
In one paragraph
Review in Nature metabolism, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20citing papers in PubMed
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Who cites it
20 citing papers in PubMed.
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- Myeloid MMP14 couples extracellular proteolysis to inflammatory and metabolic remodeling during obesity.Cell reports · 2026Article
- Tanycyte BMAL1 regulates high-fat diet weight gain and shapes arcuate neurogenesis in female mice.Cell reports · 2026Article
- Sustained loss ofbioRxiv : the preprint server for biology · 2026Article
- HuR-dependent expression of RyR2 contributes to calcium-mediated thermogenesis in murine brown adipocytes.Scientific reports · 2026Article
- Retatrutide Shows Multiple Metabolic Benefits in Diet-Induced Obese MASH Mouse and Hamster Models.Obesity (Silver Spring, Md.) · 2026Article
- Neuronal surface P antigen (NSPA) as a novel regulator of energy homeostasis and adipose tissue metabolism.Molecular medicine (Cambridge, Mass.) · 2026Article
- The Mitochondrial NAD Transporter SLC25A51 in Adipocytes Regulates Adipose Tissue Mitochondrial Function and Systemic Metabolism During Aging.Aging cell · 2026Article
- Feeding Behaviour in the KPC Model of Pancreatic Cancer-Associated Cachexia: Alteration of Spontaneous and Evoked Feeding Behaviours.Journal of cachexia, sarcopenia and muscle · 2026Article
- Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.Cell reports · 2026Article
- FGF21 signals through hindbrain neurons to alter food intake and energy expenditure during dietary protein restriction.Cell reports · 2026Article
- Differential effects of voluntary exercise and Totum-448, a plant-based formulation, in a hamster model of MASLD.Scientific reports · 2026Article
- GPR146 in adipose tissue drives adipose-liver crosstalk and promotes hepatic steatosis in mice.Nature communications · 2026Article
- Toward Scientific Rigor: Setting Standards for Indirect Calorimetry Analysis and Reporting.Diabetes · 2026Review
- On the replicability of physiological responses.The Journal of experimental biology · 2026Article
- Dietary restriction in aging and longevity.Nature aging · 2026Review
- Prediction Equations to Estimate Resting Metabolic Rate in Healthy, Community-Dwelling Chinese Older Adults.Nutrients · 2026Article
- Partial Replacement of Maltodextrin With Galactose in Post-Weaning Diets of Mice Alters Body Composition and Energy Metabolism in Early-Life and Programs Hepatic Igf1 Expression in Later-Life.Molecular nutrition & food research · 2026Article
- A Mouse Model of Lipoatrophy Reveals Relationships Between Beige Fat Appearance and Female Fertility.Endocrinology · 2025Article
Corrections and comments
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Authors and funding
80 authors.
Alexander S BanksDivision of Endocrinology, Diabetes, and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA. asbanks@bidmc.harvard.edu.ORCID http://orcid.org/0000-0003-1787-6925
David B AllisonDepartment of Epidemiology and Biostatistics, School of Public Health, Indiana University Bloomington, Bloomington, IN, USA.
Thierry AlquierCentre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montreal, Quebec, Canada.
AnsarullahThe Jackson Laboratory, Bar Harbor, ME, USA.
Steven N AustadDepartment of Biology, University of Alabama at Birmingham, Birmingham, AL, USA.
Johan AuwerxLaboratory of Integrative Systems Physiology, Institute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
Julio E AyalaDepartment of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN, USA.
Joseph A BaurDepartment of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Stefania CarobbioCentro de Investigación Principe Felipe, Valencia, Spain.
Gary A ChurchillThe Jackson Laboratory, Bar Harbor, ME, USA.
Morten DallNovo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark.
Rafael de CaboTranslational Gerontology Branch, National Institute on Aging, NIH, Baltimore, MD, USA.
Jose DonatoDepartment of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Nathalia R V DraganoInstitute of Experimental Genetics - German Mouse Clinic, Helmholtz Zentrum, Munich, Germany.
Carol F EliasDepartment of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, MI, USA.
Anthony W FerranteDepartment of Medicine, Institute of Human Nutrition, Naomi Berrie Diabetes Center, Columbia University, New York, NY, USA.
Brian N FinckCenter for Human Nutrition, Division of Nutritional Sciences and Obesity Medicine, Washington University School of Medicine, St Louis, MO, USA.
Jose E GalganiDepartamento de Nutrición y Dietética, Escuela de Ciencias de la Salud, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
Zachary Gerhart-HinesNovo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark.
Laurie J GoodyearSection on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA, USA.
Justin L GrobeDepartment of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA.
Rana K GuptaDivision of Endocrinology, Department of Medicine, Duke Molecular Physiology Institute, Duke University, Durham, NC, USA.
Kirk M HabeggerDepartment of Medicine, Division of Endocrinology, Diabetes & Metabolism, University of Alabama at Birmingham, Birmingham, AL, USA.
Sean M HartigDepartment of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA.
Andrea L HevenerDivision of Endocrinology, Diabetes and Metabolism, Department of Medicine, University of California, Los Angeles, CA, USA.
Steven B HeymsfieldPennington Biomedical Research Center, Louisiana State University, Baton Rouge, LA, USA.
Corey D HolmanInstitute for Diabetes, Obesity and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Martin Hrabě de AngelisInstitute of Experimental Genetics - German Mouse Clinic, Helmholtz Zentrum, Munich, Germany.
David E JamesThe Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.
Lawrence KazakRosalind & Morris Goodman Cancer Institute, McGill University, Montreal, Quebec, Canada.
Jae Bum KimCenter for Adipocyte Structure and Function, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
Martin KlingensporSchool of Life Sciences, Else Kröner Fresenius Center for Nutritional Medicine, ZIEL Institute for Food & Health, Technical University of Munich, Freising, Germany.
Xingxing KongSchool of Life Sciences, Fudan University, Shanghai, China.
Sander KooijmanDivision of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands.
Louise LantierDepartment of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN, USA.
K C Kent LloydDepartment of Surgery, School of Medicine, University of California, Davis, Davis, CA, USA.
James C LoWeill Center for Metabolic Health, Weill Cornell Medicine, New York, NY, USA.
Irfan J LodhiDivision of Endocrinology, Metabolism & Lipid Research, Department of Medicine, Washington University School of Medicine, Saint Louis, MO, USA.
Paul S MacLeanDivision of Endocrinology, Metabolism, and Diabetes, School of Medicine, University of Colorado Anschutz Medical Campus, Colorado, CO, USA.
Owen P McGuinnessVanderbilt Mouse Metabolic Phenotyping Center, Vanderbilt University, Nashville, TN, USA.
Gema Medina-GómezÁrea de Bioquímica y Biología Molecular, Departamento de Ciencias Básicas de la Salud, Facultad de Ciencias de la Salud, Universidad Rey Juan Carlos, Madrid, Spain.
Raghavendra G MirmiraDepartment of Medicine, The University of Chicago, Chicago, IL, USA.
Christopher D MorrisonPennington Biomedical Research Center, LSU System, Baton Rouge, LA, USA.
Gregory J MortonUniversity of Washington Medicine Diabetes Institute, Department of Medicine, Seattle, WA, USA.
Timo D MüllerInstitute for Diabetes and Obesity, Helmholtz Munich, Munich, Germany.
Yoshihiro OgawaDepartment of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
David Pajuelo-RegueraCzech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Vestec, Czechia.
Matthew J PotthoffHarold Hamm Diabetes Center, University of Oklahoma Health Sciences, Oklahoma City, OK, USA.
Nathan QiDepartment of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, MI, USA.
Marc L ReitmanDiabetes, Endocrinology, and Obesity Branch, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, USA.
Patrick C N RensenDivision of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands.
Jan RozmanLuxembourg Centre for Systems Biomedicine, University of Luxembourg, Luxembourg, Luxembourg.
Jennifer M RutkowskyDepartment of Molecular Biosciences, University of California, Davis, Davis, CA, USA.
Kei SakamotoNovo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark.
Philipp E SchererTouchstone Diabetes Center, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Gary J SchwartzDepartment of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA.
Radislav SedlacekCzech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Vestec, Czechia.
Mohammed SelloumUniversité de Strasbourg, CNRS, INSERM, CELPHEDIA, PHENOMIN, Institut Clinique de la Souris, Illkirch, France.
Saame Raza ShaikhDepartment of Nutrition, Gillings School of Global Public Health and School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Shuai ChenState Key Laboratory of Pharmaceutical Biotechnology, Model Animal Research Center, Medical School, Nanjing University, Nanjing, China.
Gerald I ShulmanDepartment of Cellular & Molecular Physiology, Yale School of Medicine, New Haven, CT, USA.
Vojtěch ŠkopDepartment of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Czechia.
Alexander A SoukasCenter for Genomic Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
John R SpeakmanSchool of Biological Sciences, University of Aberdeen, Aberdeen, UK.
Bruce M SpiegelmanDepartment of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.
Gregory R SteinbergCentre for Metabolism, Obesity and Diabetes Research, McMaster University, Hamilton, Ontario, Canada.
Katrin J SvenssonDepartment of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
John P ThyfaultDepartment of Cell Biology and Physiology, University of Kansas Medical Center, Kansas City, KS, USA.
Tony TiganisMonash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
Paul M TitchenellDepartment of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Nigel TurnerVictor Chang Cardiac Research Institute, Darlinghurst, New South Wales, Australia.
Licio A VellosoObesity and Comorbidities Research Center, University of Campinas, Campinas, Brazil.
Antonio Vidal-PuigMRC Institute of Metabolic Science and Medical Research Council, Cambridge, UK.
Christopher S WardDepartment of Integrative Physiology, Baylor College of Medicine, Houston, TX, USA.
Ashley S WilliamsDivision of Endocrinology, Department of Medicine, Duke Molecular Physiology Institute, Duke University, Durham, NC, USA.
Christian WolfrumDepartment of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland.
Allison W XuDiabetes Center, University of California, San Francisco, San Francisco, CA, USA.
Ying XuCambridge-Su Genomic Resource Center, The Fourth Affiliated Hospital, Medical School of Soochow University, Suzhou, China.
Juleen R ZierathNovo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark.
International Indirect Calorimetry Consensus Committee (IICCC)
Funding
KOMP2-Phase3 Production and Phenotyping by the DTCC Consortium TCP Transfer to UC DavisUM1OD023221 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI KC KENT LLOYD · 2016 to 2026
$45.7MWhy is the prevalence of obesity so high in U.S. Southern States? Regional predictors of BMI and obesity treatment response.P30DK056336 · NIDDK · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI BARBARA A GOWER · 2000 to 2026
$31.9MUNIV OF NORTH CAROLINA CLINICAL NUTRITION RESEARCH UNITP30DK056350 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Venkata Saroja Voruganti · 1999 to 2026
$31.6MPILOT STUDY--CLINICAL NUTRITION RESEARCHP30DK035816 · NIDDK · UNIVERSITY OF WASHINGTON · PI GREGORY J MORTON · 1986 to 2026
$30.4MRegional Pilot And Feasibility Study Grants ProgramP30DK020572 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DAVID P OLSON · 2013 to 2026
$24.3MPilot and Feasibility ProgramP30DK020595 · NIDDK · UNIVERSITY OF CHICAGO · PI RONALD N COHEN · 2013 to 2026
$20.9MStanford Islet Research CoreP30DK116074 · NIDDK · STANFORD UNIVERSITY · PI Seung K Kim · 2017 to 2026
$19.5MMouse Metabolic Phenotyping Center (MMPC) at UC DavisU2CDK092993 · NIDDK · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LLOYD, KC KENT · 2016 to 2021
$5.9MNeuroendocrine Control of Glucose MetabolismR01DK089056 · NIDDK · UNIVERSITY OF WASHINGTON · PI MORTON, GREGORY J · 2011 to 2024
$5.6MP&F programP30DK135043 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI Dennis Brown · 2023 to 2026
$5.4MThe National Center for Metabolic Phenotyping of Mouse Models of Obesity and Diabetes (MPMOD) at UC DavisU2CDK135074 · NIDDK · UNIVERSITY OF CALIFORNIA AT DAVIS · PI KC KENT LLOYD · 2023 to 2026
$4.0MRole of Polyamines and Hypusine in Nutrient-Induced Beta-Cell Growth and ReplicationR01DK124906 · NIDDK · UNIVERSITY OF CHICAGO · PI Raghavendra G Mirmira · 2020 to 2026
$4.0MAmerican Heart Association-American Stroke Association 23IPA1042031NICHD NIH HHS R01 HD106962NIDDK NIH HHS P30 DK020572NIDDK NIH HHS P30 DK020595NIDDK NIH HHS P30 DK035816NIDDK NIH HHS P30 DK056336NIDDK NIH HHS P30 DK056350NIDDK NIH HHS P30 DK116074NIDDK NIH HHS P30 DK135043NIDDK NIH HHS R01 DK089056NIDDK NIH HHS R01 DK124906NIDDK NIH HHS R01 DK125260NIDDK NIH HHS R01 DK133948NIDDK NIH HHS RC2 DK142612NIDDK NIH HHS U2C DK092993NIDDK NIH HHS U2C DK134901NIDDK NIH HHS U2C DK135066NIDDK NIH HHS U2C DK135073NIDDK NIH HHS U2C DK135074NIH HHS S10 OD028635NIH HHS UM1 OD023221
Abstract
Understanding the complex factors influencing mammalian metabolism and body weight homeostasis is a long-standing challenge requiring knowledge of energy intake, absorption and expenditure. Using measurements of respiratory gas exchange, indirect calorimetry can provide non-invasive estimates of whole-body energy expenditure. However, inconsistent measurement units and flawed data normalization methods have slowed progress in this field. This guide aims to establish consensus standards to unify indirect calorimetry experiments and their analysis for more consistent, meaningful and reproducible results. By establishing community-driven standards, we hope to facilitate data comparison across research datasets. This advance will allow the creation of an in-depth, machine-readable data repository built on shared standards. This overdue initiative stands to markedly improve the accuracy and depth of efforts to interrogate mammalian metabolism. Data sharing according to established best practices will also accelerate the translation of basic findings into clinical applications for metabolic diseases afflicting global populations.
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Calorimetry, IndirectAnimalsEnergy MetabolismHumans
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