Evidence map›Paper›PMID 40993149›Full record

ArticleNature communications2025

Mechanically activated snai1b coordinates the initiation of myocardial delamination for trabeculation.

Jing Wang, Aaron L Brown, Seul-Ki Park, Charlie Z Zheng, Adam Langenbacher, Enbo Zhu, Ryan O'Donnell, Peng Zhao, Jeffrey J Hsu, Tomohiro Yokota and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Jing WangDepartment of Bioengineering, University of California, Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0002-3203-5131
Aaron L BrownDepartment of Mechanical Engineering, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-1301-6189
Seul-Ki ParkDivision of Cardiology, Department of Medicine, School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Charlie Z ZhengDepartment of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0001-8934-8967
Adam LangenbacherDepartment of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA.
Enbo ZhuDivision of Cardiology, Department of Medicine, School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0001-8600-8553
Ryan O'DonnellDivision of Cardiology, Department of Medicine, School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Peng ZhaoDivision of Cardiology, Department of Medicine, School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0002-6610-6217
Jeffrey J HsuDivision of Cardiology, Department of Medicine, School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Tomohiro YokotaDivision of Cardiology, Department of Medicine, School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Jiandong LiuDepartment of Pathology and Laboratory Medicine, McAllister Heart Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.ORCID http://orcid.org/0000-0003-0035-3570
Jau-Nian ChenDepartment of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0001-8807-3607
Alison L MarsdenDepartments of Pediatrics and Bioengineering, Stanford University, Stanford, CA, USA.
Tzung K HsiaiDepartment of Bioengineering, University of California, Los Angeles, Los Angeles, CA, USA. thsiai@mednet.ucla.edu.ORCID http://orcid.org/0000-0003-1734-0792

Funding

Shear Stress and Light-Sheets to Study Cardiac TrabeculationR01HL129727 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HSIAI, TZUNG K, MARSDEN, ALISON L · 2015 to 2024
$3.5M
Macrophage functional dynamics in adult heart regenerationR01HL164933 · NHLBI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Jiandong Liu · 2023 to 2026
$2.4M
Kilohertz 3D Optical Mapping of Atrial Fibrillation in Beating Zebrafish HeartsR01HL165318 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GAO, LIANG, HSIAI, TZUNG K · 2022 to 2025
$2.2M
Integrating Volumetric Light-Field with Computational Fluid Dynamics to Study Myocardial Trabeculation and FunctionR01HL159970 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HSIAI, TZUNG K, MARSDEN, ALISON L · 2021 to 2024
$2.0M
UCLA and Caltech integrated Cardiovascular Medicine for Bioengineers (iCMB)T32HL144449 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HSIAI, TZUNG K, PAJUKANTA, PAIVI · 2020 to 2024
$1.2M
NHLBI NIH HHS R01 HL129727NHLBI NIH HHS R01 HL159970NHLBI NIH HHS R01 HL164933NHLBI NIH HHS R01 HL165318NHLBI NIH HHS T32 HL144449U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI) R01HL129727U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI) R01HL159970U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI) R01HL165318U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI) T32HL144449
6 · The paper itself

Abstract

During development, myocardial contractile force and intracardiac hemodynamic shear stress coordinate the initiation of trabeculation. While Snail family genes are well-recognized transcription factors of epithelial-to-mesenchymal transition, snai1b-positive cardiomyocytes are sparsely distributed in the ventricle of zebrafish at 4 days post-fertilization. Isoproterenol treatment significantly increases the number of snai1b-positive cardiomyocytes, of which 80% are Notch-negative. CRISPR-activation of snai1b leads to 51.6% cardiomyocytes forming trabeculae, whereas CRISPR-repression reduces trabecular cardiomyocytes to 6.7% under isoproterenol. In addition, 36.7% of snai1b-repressed cardiomyocytes undergo apical delamination. 4-D strain analysis demonstrates that isoproterenol increases the myocardial strain along radial trabecular ridges in alignment with the snai1b expression and Notch-ErbB2-mediated trabeculation. Single-cell and spatial transcriptomics reveal that these snai1b-positive cardiomyocytes are devoid of some epithelial-to-mesenchymal transition-related phenotypes, such as Col1a2 production and induction by ErbB2 or TGF-β. Thus, we uncover snai1b-positive cardiomyocytes that are mechanically activated to initiate delamination for cardiac trabeculation.

Indexed as

HeartMechanotransduction, CellularMyocytes, CardiacSnail Family Transcription FactorsAnimalsGene Expression Regulation, DevelopmentalMyocardial ContractionZebrafishZebrafish Proteinssnai1b protein, zebrafishSnail Family Transcription FactorsZebrafish Proteins

Identifiers

PMID40993149
PMCPMC12460811

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.