Evidence map›Paper›PMID 40993113›Full record

ArticleNature communications2025

Three lncRNAs promote PUM protein condensation and germline differentiation.

Roni Falk, Noa Gilad, Hanna Achache, Yisrael Rappaport, Roni Halfon, Reut Shabtai, Hasan Ishtayeh, Laura Wolovelsky, Oren Ram, Yonatan B Tzur

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Roni FalkDepartment of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Noa GiladDepartment of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Hanna AchacheDepartment of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Yisrael RappaportDepartment of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Roni HalfonDepartment of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Reut ShabtaiDepartment of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Hasan IshtayehDepartment of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Laura WolovelskyDepartment of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Oren RamDepartment of Biological Chemistry, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Yonatan B TzurDepartment of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel. tzur@mail.huji.ac.il.ORCID http://orcid.org/0000-0002-7715-6113

Funding

EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council) 15260Israel Science Foundation (ISF) 979/21United States - Israel Binational Science Foundation (BSF) 2023036
6 · The paper itself

Abstract

Long non-coding RNAs (lncRNAs) are abundant in gonads, yet most knockout models show no fertility defects, leaving their role unclear. Here, we identify overlapping functions of three lncRNAs in Caenorhabditis elegans fertility. These lncRNAs bind and sequester FBF-2, a regulator that inhibits germ cell differentiation. Combined deletion of the lncRNAs reduces progenitor cell number and overall progeny, reflecting additive effects. In lncRNA mutants, pro-meiotic transcripts-normally destabilized by FBF-2-are significantly reduced. FBF-2 localizes to both the cytoplasm and peri-nuclear condensates in germ cells, but its peri-nuclear condensation and association with P granules-phase-separated, evolutionarily conserved structures-are diminished in lncRNA mutants. Our findings suggest that these lncRNAs cooperatively promote fertility by spatially restricting FBF-2 within granules, thus reducing its activity and allowing the expression of differentiation-promoting transcripts. The requirement for multiple knockouts to reveal this phenotype highlights the redundancy and combinatorial function of lncRNAs in regulating germline development.

Indexed as

Caenorhabditis elegansCaenorhabditis elegans ProteinsCell DifferentiationGerm CellsRNA-Binding ProteinsRNA, Long NoncodingAnimalsFertilityGene Expression Regulation, DevelopmentalMutationCaenorhabditis elegans ProteinsRNA-Binding ProteinsRNA, Long Noncoding

Identifiers

PMID40993113
PMCPMC12460645

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.