Evidence map›Paper›PMID 40992837›Full record

ArticleMethods in enzymology2025

Development of a flow cytometric method to evaluate the impact of N-terminal sequences on protein stability.

Sabyasachi Sen, Khai Khee Kho, Aditya M Kunjapur

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sabyasachi SenDepartment of Chemical & Biomolecular Engineering, University of Delaware, Newark, DE, United States.
Khai Khee KhoDepartment of Chemical & Biomolecular Engineering, University of Delaware, Newark, DE, United States.
Aditya M KunjapurDepartment of Chemical & Biomolecular Engineering, University of Delaware, Newark, DE, United States. Electronic address: kunjapur@udel.edu.

Funding

This renovation project will create over 1455 sq. ft. of state- of-the-art reseaP20GM104316 · NIGMS · UNIVERSITY OF DELAWARE · PI BIRD, JEREMY GILBERT · 2014 to 2024
$26.8M
Boosting efficacy of oral vaccine candidates by enabling spore display of nitrated antigensDP2AI176137 · NIAID · UNIVERSITY OF DELAWARE · PI KUNJAPUR, ADITYA MOHAN · 2022 to 2025
$2.4M
NIAID NIH HHS DP2 AI176137NIGMS NIH HHS P20 GM104316
6 · The paper itself

Abstract

Protein library generation coupled with high-throughput analysis has become an increasingly mainstream method to study the contribution of N-terminal regions to protein stability. Given this trend, the establishment of experimental best practices would benefit the field. In this chapter, we present a method for the generation and screening of N-terminal libraries using a flow cytometric method that pairs Fluorescence-Activated Cell Sorting (FACS) with Next Generation Sequencing (NGS). We demonstrate the ability of our workflow to identify a stabilizing effect imparted by acidic residues in the third position of an N-degron within the model microbe Escherichia coli. To conclude, we comment on the applications and generalizability of this technology for screening clustered mutagenized regions.

Indexed as

Escherichia coli ProteinsFlow CytometryEscherichia coliHigh-Throughput Nucleotide SequencingPeptide LibraryProtein StabilityEscherichia coli ProteinsPeptide LibraryEscherichia coliFlow cytometryFluorescence-activated cell sortingN-degron pathwayNext-generation sequencingProtein stability

Identifiers

PMID40992837
PMCPMC13649343

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.