ArticlePloS one2025
The function of Anr in the differential effects of oxygen levels on biofilm development and nitrogenase performance in Pseudomonas stutzeri A1501.
Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- An engineered Pseudomonas stutzeri strain overexpressing the sigma factor AlgU promotes maize growth under salt stress.Applied microbiology and biotechnology · 2026Article
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6 authors.
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Abstract
Pseudomonas stutzeri A1501 exhibits a rare and notable trait: nitrogenase activity, which functions under microaerophilic conditions with limited oxygen availability. Optimal biofilm formation occurs in minimal media under nitrogen-depleted conditions. Anr is a transcription regulator with a widespread influence that accelerates the process of biofilm development. The lack of Anr adversely affects nitrogen fixation by regulating the activity of nifA, nifH, and ntrC. The anr insertion mutant significantly reduced the nitrogenase activity. Nitrogenase activity demonstrated considerable variation at different oxygen concentrations. The quantitative reverse transcription polymerase chain reaction (qRT-PCR) demonstrated a reduction in the expression of nif island genes during nitrogen fixation in the absence of anr gene. The discovery revealed that different oxygen levels in the environment significantly influence nitrogenase activity and biofilm formation. The qRT-PCR investigation demonstrated an upregulation of narL gene expression during biofilm formation, suggesting that reduced oxygen levels initiate a signaling cascade that activates the anr gene. This influences both the expression of RpoS (sigma factor) genes and the process of biofilm formation.
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