Evidence map›Paper›PMID 40991099›Full record

ArticleProbiotics and antimicrobial proteins2026

Three Characterized Probiotics from Dairy Sources-Weissella confusa (ProY 5.1), Enterococcus lactis (ProY 5.3), and Leuconostoc lactis (RM 2.1)-Exhibit Potential for Antidiarrheal Effects.

Jannatul Ferdous, Md Saddam Hossain, Palash Kumar Sarker, Md Shimul Bhuia, Md Sakib Al Hasan, Raihan Chowdhury, Md Sarafat Ali, Md Robiul Auwal, Keshob Chandra Das, Muhammad Torequl Islam and 2 more

Abstract read
PubMed Publisher
In one paragraph

Article in Probiotics and antimicrobial proteins, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jannatul Ferdous *Microbial Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, 1349, Bangladesh.
Md Saddam Hossain *Microbial Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, 1349, Bangladesh.
Palash Kumar SarkerMicrobial Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, 1349, Bangladesh.
Md Shimul BhuiaBioinformatics and Drug Innovation Laboratory, BioLuster Research Center Ltd., Gopalganj, 8100, Bangladesh.
Md Sakib Al HasanBioinformatics and Drug Innovation Laboratory, BioLuster Research Center Ltd., Gopalganj, 8100, Bangladesh.
Raihan ChowdhuryBioinformatics and Drug Innovation Laboratory, BioLuster Research Center Ltd., Gopalganj, 8100, Bangladesh.
Md Sarafat AliDepartment of Biotechnology and Genetic Engineering, Gopalganj Science and Technology University, Gopalganj, 8100, Bangladesh.
Md Robiul AuwalMicrobial Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, 1349, Bangladesh.
Keshob Chandra DasMolecular Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, 1349, Bangladesh.
Muhammad Torequl IslamBioinformatics and Drug Innovation Laboratory, BioLuster Research Center Ltd., Gopalganj, 8100, Bangladesh.
Abu HashemMicrobial Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, 1349, Bangladesh. hashemnib04@yahoo.com.
Md SalimullahMolecular Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, 1349, Bangladesh. salim2969@gmail.com.

Funding

Science and Technology Special Research Grant, Bangladesh SRG-231229 and SRG-241142
6 · The paper itself

Abstract

Probiotics are beneficial live microorganisms that may provide secure management of several diseases, such as diarrhea. The current research aims to assess the antidiarrheal activity of potential probiotics by utilizing in vitro and in vivo approaches. Bacterial strains were isolated from dairy products and screened for probiotic characterization, with four strains, viz., Weissella confusa (ProY 5.1), Enterococcus lactis (ProY 5.3), Leuconostoc lactis (RM 2.1), and Bacillus subtilis (ProY 11.3), selected based on in vitro approaches. These strains demonstrated non-hemolytic properties, acidic resistance, tolerance to bile salt and phenol, strong adherence rate, probiotic activity, and antimicrobial activity against six enteric pathogens. Potential strains were identified by applying molecular techniques: MALDI-TOF and whole genome sequencing. In vivo experiments on castor oil-induced diarrheal Swiss albino mice showed a positive impact when compared to loperamide and bismuth subsalicylate as positive controls, whereas the PBS + MRS broth was used as a negative control. Screened ProY 5.1, ProY 5.3, and RM 2.1 significantly lengthened latency periods and reduced diarrheal symptoms (p < 0.005). Comparatively low antidiarrheal activity was found in the ProY 11.3 isolate. The combination of candidate probiotics exhibited stronger activity compared to the individual ones. In conclusion, the three strains might be potential probiotic candidates for managing diarrhea.

Indexed as

AntidiarrhealsDairy ProductsDiarrheaEnterococcusLeuconostocProbioticsWeissellaAnimalsCastor OilFemaleMiceAntidiarrhealsCastor OilAntidiarrheal activityCastor oilEnterococcus lactisLeuconostoc lactisProbioticsWeissella confusa

Identifiers

PMID40991099

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.