ArticleJournal of animal science2025
Zinc-loaded montmorillonite alleviates avian pathogenic Escherichia coli-induced intestinal barrier damage in broiler chickens.
Article in Journal of animal science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed.
- Article
- Rosmarinic Acid Ameliorates PMAnimals : an open access journal from MDPI · 2026Article
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7 authors.
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Abstract
Avian pathogenic Escherichia coli (APEC) significantly compromises the intestinal barrier of broiler chickens. This study evaluated the protective effects of zinc-loaded montmorillonite (Zn-MMT), an inorganic antibacterial compound, against APEC challenge in broiler chickens. A total of 180 1-d-old male Arbor Acres broilers were randomly divided into 3 groups with 6 replicates of 10 birds each. The treatment groups were: 1) the control group (basal diet); 2) the APEC-challenged group (basal diet); and 3) the Zn-MMT-supplemented group (basal diet + 1,000 mg/kg Zn-MMT). On day 14, groups 2 and 3 were orally challenged with APEC, while controls received sterile broth. Intergroup differences were assessed using one-way ANOVA and Tukey's multiple comparison test. Compared with the APEC-challenged group, dietary Zn-MMT supplementation reduced myeloperoxidase activity in both jejunal and ileal mucosa and increased the relative weight of the small intestine (duodenum, jejunum, and ileum) (P < 0.05). Moreover, Zn-MMT lowered serum endotoxin and D-lactate concentrations, as well as diamine oxidase activity, while increasing jejunal villus height and the ratio between villus height and crypt depth (P < 0.05). The incorporation of Zn-MMT upregulated the expression of jejunal zonula occludens-1 and claudin-1 and ileal zonula occludens-1 (P < 0.05). Additionally, Zn-MMT decreased the apoptosis rates of the epithelial cells in the jejunum and ileum (P < 0.05). The Zn-MMT supplementation restored the expression of cysteine-requiring aspartate protease-3, cysteine-requiring aspartate protease-9, B cell lymphoma-2, and tumor protein 53 in the jejunum and/or ileum of the APEC-challenged chickens (P < 0.05). Dietary supplementation with Zn-MMT improved antioxidant capacity of APEC-challenged chickens by increasing the activities of superoxide dismutase and catalase, elevating reduced glutathione levels, and inhibiting the accumulation of malondialdehyde in serum and/or intestinal mucosa (P < 0.05). The results suggested that Zn-MMT was effective in alleviating intestinal barrier damage in APEC-challenged broiler chickens by alleviating inflammation, reducing intestinal permeability, regulating tight junction protein expression, preventing intestinal apoptosis, modulating apoptosis-related genes, and mitigating oxidative stress.
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