Evidence map›Paper›PMID 40987911›Full record

ArticleFolia microbiologica2025

Exploring the impact of quercetin on the growth and biofilm formation of Leptospira interrogans.

Elumalai Rajalakshmi, Evangeline Seles J L, Anandkumar Balakrishnan, Kakithakara Vajravelu Leela, Mohandass Ramya

Abstract read
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In one paragraph

Article in Folia microbiologica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Elumalai Rajalakshmi *Molecular Genetics Laboratory, Department of Genetic Engineering, School of Bioengineering, Faculty of Engineering and Technology, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu, Tamil Nadu, 603203, India.ORCID http://orcid.org/0009-0000-9802-4286
Evangeline Seles J L *Molecular Genetics Laboratory, Department of Genetic Engineering, School of Bioengineering, Faculty of Engineering and Technology, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu, Tamil Nadu, 603203, India.
Anandkumar BalakrishnanCorrosion Science and Technology Division, Metallurgy and Materials Group, Indira Gandhi Centre for Atomic Research, Kalpakkam, 603102, Tamil Nadu, India.
Kakithakara Vajravelu LeelaDepartment of Microbiology, SRM Medical College Hospital and Research Centre, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu, Chennai, Tamil Nadu, 603 203, India.
Mohandass RamyaMolecular Genetics Laboratory, Department of Genetic Engineering, School of Bioengineering, Faculty of Engineering and Technology, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu, Tamil Nadu, 603203, India. ramya.mohandass@gmail.com.ORCID http://orcid.org/0000-0003-4448-920X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Leptospira interrogans, the causative agent of leptospirosis, presents major challenges due to its ability to form environmental biofilms, persistent virulence, and emerging antimicrobial resistance. Plant-derived bioactive compounds offer promising alternatives to conventional antibiotics in targeting bacterial biofilms. This study investigates the antibiofilm and antimicrobial potential of quercetin, a plant-derived polyphenol, against L. interrogans. Quercetin significantly inhibited biofilm formation (91%), induced 44% biofilm dispersion at 500 µg/mL, and reduced viable cell counts by 92% at 1000 µg/mL compared to the untreated control. These effects were further validated using fluorescence-activated cell sorting (FACS), which demonstrated reduction in the live cells in the presence of quercetin. The expression levels of key genes associated with virulence and biofilm regulation were assessed using quantitative RT-PCR, revealing downregulation of csrA and upregulation of lipL32 in quercetin-treated cells. Potential molecular interactions between several leptospiral proteins and quercetin were proven through AutoDock Vina and PyMOL. These findings highlight quercetin's potential as an anti-leptospiral agent for managing leptospiral biofilm formation and dispersions.

Indexed as

BiofilmsL. interrogansMolecular dockingQuercetin

Identifiers

PMID40987911

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.