Evidence map›Paper›PMID 40987870›Full record

ArticleNature methods2025

Kilohertz volumetric imaging of in vivo dynamics using squeezed light field microscopy.

Zhaoqiang Wang, Ruixuan Zhao, Daniel A Wagenaar, Diego Espino, Liron Sheintuch, Ohr Benshlomo, Wenjun Kang, Enbo Zhu, Calvin K Lee, William C Schmidt and 10 more

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Dual-Channel Event Microscopy for Ultrafast Biological Imaging.bioRxiv : the preprint server for biology · 2025
    Article
  7. Article
  8. Article
  9. Review
  10. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors.

Zhaoqiang Wang *Department of Bioengineering, Henry Samueli School of Engineering and Applied Science, University of California, Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0002-6507-6167
Ruixuan Zhao *Department of Bioengineering, Henry Samueli School of Engineering and Applied Science, University of California, Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0009-0002-2260-203X
Daniel A WagenaarDivision of Biology and Biological Engineering, Tianqiao and Chrissy Chen Institute for Neuroscience, California Institute of Technology, Pasadena, CA, USA.ORCID http://orcid.org/0000-0002-6222-761X
Diego EspinoDepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Liron SheintuchDepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0003-4245-5786
Ohr BenshlomoDepartment of Bioengineering, Henry Samueli School of Engineering and Applied Science, University of California, Los Angeles, Los Angeles, CA, USA.
Wenjun KangWyant College of Optical Science, University of Arizona, Tucson, AZ, USA.ORCID http://orcid.org/0000-0003-1486-7249
Enbo ZhuDivision of Cardiology, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0001-8600-8553
Calvin K LeeDepartment of Bioengineering, Henry Samueli School of Engineering and Applied Science, University of California, Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0001-6789-0317
William C SchmidtDepartment of Bioengineering, Henry Samueli School of Engineering and Applied Science, University of California, Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0001-9780-9495
Aryan PammarDepartment of Bioengineering, Henry Samueli School of Engineering and Applied Science, University of California, Los Angeles, Los Angeles, CA, USA.
Jing WangDivision of Cardiology, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Gerard C L WongDepartment of Bioengineering, Henry Samueli School of Engineering and Applied Science, University of California, Los Angeles, Los Angeles, CA, USA.
Rongguang LiangWyant College of Optical Science, University of Arizona, Tucson, AZ, USA.
Sungmoo LeeDepartment of Neurobiology, Stanford University, Stanford, CA, USA.
Michael Z LinDepartments of Neurobiology and Bioengineering; Department of Chemical & Systems Biology; Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA, USA.
Madhuvanthi KannanDepartment of Neuroscience, University of Minnesota, Minneapolis, MN, USA.
Peyman GolshaniDepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0002-5406-7695
Tzung K HsiaiDivision of Cardiology, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0003-1734-0792
Liang GaoDepartment of Bioengineering, Henry Samueli School of Engineering and Applied Science, University of California, Los Angeles, Los Angeles, CA, USA. gaol@ucla.edu.ORCID http://orcid.org/0000-0002-4296-5586

Funding

MULTIDISCIPLINARY TRAINING IN MICROBIAL PATHOGENESIST32AI007323 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Peter John Bradley · 1988 to 2026
$8.6M
Vibrio cholerae c-diGMP signaling: Motile to biofilm transition and transmissionR01AI102584 · NIAID · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI YILDIZ, HAVVA FITNAT · 2013 to 2023
$6.5M
Shear Stress and Light-Sheets to Study Cardiac TrabeculationR01HL129727 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HSIAI, TZUNG K, MARSDEN, ALISON L · 2015 to 2024
$3.5M
Ultrafast BioimagingR35GM128761 · NIGMS · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI Liang Gao · 2018 to 2026
$3.2M
Intravascular Deployment of a Wirelessly Powered Micro-PacerR01HL149808 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Tzung K Hsiai · 2020 to 2026
$2.8M
Kilohertz 3D Optical Mapping of Atrial Fibrillation in Beating Zebrafish HeartsR01HL165318 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GAO, LIANG, HSIAI, TZUNG K · 2022 to 2025
$2.2M
Integrating Volumetric Light-Field with Computational Fluid Dynamics to Study Myocardial Trabeculation and FunctionR01HL159970 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HSIAI, TZUNG K, MARSDEN, ALISON L · 2021 to 2024
$2.0M
Kilohertz volumetric imaging of neuronal action potentials in awake behaving miceRF1NS128488 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GAO, LIANG, GOLSHANI, PEYMAN · 2022 to 2022
$1.6M
UCLA and Caltech integrated Cardiovascular Medicine for Bioengineers (iCMB)T32HL144449 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HSIAI, TZUNG K, PAJUKANTA, PAIVI · 2020 to 2024
$1.2M
Kilohertz 3D voltage imaging using near-infrared confocal squeezed light field microscopyR01NS142690 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Liang Gao · 2025 to 2026
$1.1M
Division of Intramural Research, National Institute of Allergy and Infectious Diseases (Division of Intramural Research of the NIAID) R01AI102584NHLBI NIH HHS R01 HL129727NHLBI NIH HHS R01 HL149808NHLBI NIH HHS R01 HL159970NHLBI NIH HHS R01 HL165318NHLBI NIH HHS T32 HL144449NIAID NIH HHS R01 AI102584NIAID NIH HHS T32 AI007323NIGMS NIH HHS R35 GM128761NINDS NIH HHS R01 NS142690NINDS NIH HHS RF1 NS128488U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI) 5T32HL144449U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI) R01HL129727U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI) R01HL159970U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI) R01HL165318U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) R35GM128761U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) RF1NS128488
6 · The paper itself

Abstract

Volumetric functional imaging of transient cellular signaling and motion dynamics is often limited by hardware bandwidth and the scarcity of photons under short exposures. To overcome these challenges, we introduce squeezed light field microscopy (SLIM), a computational imaging approach that rapidly captures high-resolution three-dimensional light signals using only a single, low-format camera sensor. SLIM records over 1,000 volumes per second across a 550-µm diameter field of view and 300-µm depth, achieving 3.6-µm lateral and 6-µm axial resolution. Here we demonstrate its utility in blood cell velocimetry within the embryonic zebrafish brain and in freely moving tails undergoing high-frequency swings. Millisecond-scale temporal resolution further enables precise voltage imaging of neural membrane potentials in the leech ganglion and hippocampus of behaving mice. Together, these results establish SLIM as a versatile and robust tool for high-speed volumetric microscopy across diverse biological systems.

Indexed as

Imaging, Three-DimensionalMicroscopyAnimalsBrainHippocampusLeechesMiceTailZebrafish

Identifiers

PMID40987870
PMCPMC12832183

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.