ArticleAnnals of laboratory medicine2026
Development and Validation of a High-performance Liquid Chromatography-Tandem Mass Spectrometry Method for Detecting Adrenocortical Hormones and Establishment of Age-stratified Reference Intervals in Reproductive-aged Women from Guangxi, China.
Yixuan Liu, Tingwei Jin, Yushuang Wei, Xuelian Qin, Siyu Deng, Jie Zheng, Boteng Yan, Yuanyuan Nong, Yu Ye, Shengzhu Huang and 8 more
Abstract readValidation Study
In one paragraphArticle in Annals of laboratory medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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18 authors.
Yixuan LiuCollaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed by the Province and Ministry, Guangxi Medical University, Nanning, China.ORCID https://orcid.org/0009-0008-0832-5009 Tingwei JinCenter for Genomic and Personalized Medicine, Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Guangxi Medic.ORCID https://orcid.org/0009-0004-8051-0306 Yushuang WeiCenter for Genomic and Personalized Medicine, Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Guangxi Medic.ORCID https://orcid.org/0009-0009-4099-0989 Siyu DengCenter for Genomic and Personalized Medicine, Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Guangxi Medic.ORCID https://orcid.org/0009-0002-6664-7299 Jie ZhengCenter for Genomic and Personalized Medicine, Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Guangxi Medic.ORCID https://orcid.org/0000-0002-8250-7061 Boteng YanCenter for Genomic and Personalized Medicine, Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Guangxi Medic.ORCID https://orcid.org/0009-0005-1247-1541 Yuanyuan NongCenter for Genomic and Personalized Medicine, Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Guangxi Medic.ORCID https://orcid.org/0009-0006-9487-7058 Yu YeCenter for Genomic and Personalized Medicine, Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Guangxi Medic.ORCID https://orcid.org/0000-0002-7025-436X Shengzhu HuangCenter for Genomic and Personalized Medicine, Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Guangxi Medic.ORCID https://orcid.org/0000-0002-0956-5097 Ganqin WangCenter for Genomic and Personalized Medicine, Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Guangxi Medic.ORCID https://orcid.org/0009-0009-8534-6476 Zengnan MoCenter for Genomic and Personalized Medicine, Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Guangxi Medic.ORCID https://orcid.org/0000-0002-3047-3138 Yonghua JiangCenter for Genomic and Personalized Medicine, Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Guangxi Medic.ORCID https://orcid.org/0000-0003-2927-4921 Funding
No grant is acknowledged in the PubMed record.
6 · The paper itselfAbstract
Background: Adrenocortical hormones, particularly 11-oxygenated androgens, are pivotal in female reproductive health and fertility. Standardized detection kits and population-specific reference intervals are lacking in China, hindering related clinical applications. Methods: A HPLC-tandem mass spectrometry (HPLC-MS/MS) pipeline was developed, rigorously validated, and applied to simultaneously quantify corticosterone, cortisone, cortisol, 18-OH cortisol, androstenedione (A4), 11β-hydroxyandrostenedione (11-OH A4), dehydroepiandrosterone, and dehydroepiandrosterone sulfate in serum samples from 455 reproductive-aged women (18-45 yrs) in Guangxi, China. Age-dependent concentration trends were analyzed, and reference intervals stratified by age (2.5th to 97.5th percentiles) were established. Correlations with body-composition metrics, ethnicity, and the menstrual cycle were investigated. Results: The HPLC-MS/MS method demonstrated high precision (intra- and inter-assay CVs <15%), accuracy, and sensitivity. All eight hormones exhibited significant age-related declines ( Conclusions: We developed an HPLC-MS/MS-based method for simultaneously quantifying eight adrenocortical hormones, including 11-OH A4, and defined age-specific reference intervals for reproductive-aged Chinese women. These findings advance the clinical utility of adrenocortical hormones in diagnosing and managing reproductive disorders.
Indexed as
Adrenal Cortex HormonesTandem Mass SpectrometryAdolescentAdultAge FactorsAndrostenedioneChinaChromatography, High Pressure LiquidDehydroepiandrosterone SulfateFemaleHumansHydrocortisoneMiddle AgedReference ValuesYoung AdultAdrenal Cortex HormonesAndrostenedioneDehydroepiandrosterone SulfateHydrocortisone11-Oxygenated androgensAdrenocortical hormonesAge-stratified reference intervalsHigh-performance liquid chromatography-tandem mass spectrometry
Identifiers
PMID40987612
PMCPMC12915376
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