Evidence map›Paper›PMID 40986198›Full record

ArticleBiotechnology letters2025

A novel approach for the in vitro assay of phospholipase C and its application in analyzing the effect of calcium and pH on populus PsnPLC.

Yao Sun, Chunhui Yang, Xin Sun, Yao Li, Qiong Wu, Di Fu, Lei Wang

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Article in Biotechnology letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yao SunInstitute of Advanced Technology, Heilongjiang Academy of Sciences, Harbin, 150020, China. f3z21@163.com.ORCID http://orcid.org/0000-0002-4061-1653
Chunhui YangInstitute of Advanced Technology, Heilongjiang Academy of Sciences, Harbin, 150020, China.
Xin SunInstitute of Advanced Technology, Heilongjiang Academy of Sciences, Harbin, 150020, China.
Yao LiInstitute of Advanced Technology, Heilongjiang Academy of Sciences, Harbin, 150020, China.
Qiong WuInstitute of Advanced Technology, Heilongjiang Academy of Sciences, Harbin, 150020, China.
Di FuInstitute of Advanced Technology, Heilongjiang Academy of Sciences, Harbin, 150020, China.
Lei WangInstitute of Advanced Technology, Heilongjiang Academy of Sciences, Harbin, 150020, China.

Funding

Excellent Youth Foundation of Heilongjiang Academy of Sciences CXJQ2021GJS02National Natural Science Foundation of China 32301622Pre-research Project of Heilongjiang Academy of Sciences YYBZ2024GJS01
6 · The paper itself

Abstract

purposePhosphoinositide-specific phospholipase C plays a crucial role in the trans-membrane and lipid signal transduction. Previous studies have elucidated the biological functions of PI-PLC. However, its function mechanism has not been fully understood.

resultsWe present a novel approach to analyze the in vitro activity of phospholipase C from Populus simonii x P. nigra. The results indicate that the optimal condition for PsnPLC activity is pH 7.4/10 μM Ca

conclusionThe findings identify Ca

Indexed as

CalciumPlant ProteinsPopulusType C PhospholipasesHydrogen-Ion ConcentrationCalciumPlant ProteinsType C PhospholipasesC2 domainCalciumEF-hand domainElectrophoresis migration shift assayIn vitro activityPHPhosphoinositide-specific phospholipase C

Identifiers

PMID40986198

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.