ArticleJournal of experimental botany2025
Revealing plasma membrane protein dynamics in living plant cells with single-molecule tracking.
Article in Journal of experimental botany, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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4 authors.
Funding
Abstract
The behavior of proteins and other biomolecules in cellular environments is governed by complex molecular processes. Understanding their temporal dynamics and interactions with other biomolecules or cellular structures is essential for elucidating physiological functions. Single-particle tracking photoactivated localization microscopy (sptPALM) has emerged as a powerful single-molecule technique for investigating these processes with exceptional spatial and temporal resolution. In this Expert View, we introduce sptPALM and focus on its application in the plasma membrane of plant cells. Key aspects and advances in the technique, including instrumentation and data analysis, are discussed to equip researchers with the foundational knowledge required to establish and execute sptPALM experiments. Recent studies are highlighted to demonstrate the potential of sptPALM to advance our understanding of molecular dynamics in plant cells.
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Registered trials
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